Heterozygous Che-1 KO mice show deficiencies in object recognition memory persistence

Gisela Zalcman, Nicoletta Corbi, Maria Grazia Di Certo, Elisabetta Mattei, Noel Federman, Arturo Romano

Research output: Contribution to journalArticlepeer-review


Transcriptional regulation is a key process in the formation of long-term memories. Che-1 is a protein involved in the regulation of gene transcription that has recently been proved to bind the transcription factor NF-κB, which is known to be involved in many memory-related molecular events. This evidence prompted us to investigate the putative role of Che-1 in memory processes. For this study we newly generated a line of Che-1+/− heterozygous mice. Che-1 homozygous KO mouse is lethal during development, but Che-1+/− heterozygous mouse is normal in its general anatomical and physiological characteristics. We analyzed the behavioral characteristic and memory performance of Che-1+/− mice in two NF-κB dependent types of memory. We found that Che-1+/− mice show similar locomotor activity and thigmotactic behavior than wild type (WT) mice in an open field. In a similar way, no differences were found in anxiety-like behavior between Che-1+/− and WT mice in an elevated plus maze as well as in fear response in a contextual fear conditioning (CFC) and object exploration in a novel object recognition (NOR) task. No differences were found between WT and Che-1+/− mice performance in CFC training and when tested at 24 h or 7 days after training. Similar performance was found between groups in NOR task, both in training and 24 h testing performance. However, we found that object recognition memory persistence at 7 days was impaired in Che-1+/− heterozygous mice. This is the first evidence showing that Che-1 is involved in memory processes.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalNeuroscience Letters
Publication statusPublished - Oct 6 2016


  • Che-1
  • Fear conditioning
  • Gene transcription
  • Long-term memory
  • Memory persistence
  • Mouse
  • Novel object recognition

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)


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