TY - JOUR
T1 - Herpes simplex virus type-specific antibody determination by enzyme-linked immunosorbent assay in human sera
AU - Revello, Maria Grazia
AU - Gerna, Giuseppe
PY - 1988
Y1 - 1988
N2 - Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.
AB - Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.
KW - ELISA
KW - HSV antibody typing
KW - HSV strain typing
KW - microneutralization
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U2 - 10.1016/0888-0786(88)90006-6
DO - 10.1016/0888-0786(88)90006-6
M3 - Article
AN - SCOPUS:38249029449
SN - 0888-0786
VL - 2
SP - 415
EP - 422
JO - Serodiagnosis and Immunotherapy in Infectious Disease
JF - Serodiagnosis and Immunotherapy in Infectious Disease
IS - 6
ER -