TY - JOUR
T1 - From hundreds to thousands
T2 - Widening the normal human Urinome
AU - Santucci, Laura
AU - Candiano, Giovanni
AU - Petretto, Andrea
AU - Bruschi, Maurizio
AU - Lavarello, Chiara
AU - Inglese, Elvira
AU - Giorgio Righetti, Pier
AU - Marco Ghiggeri, Gian
PY - 2014/12/1
Y1 - 2014/12/1
N2 - The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name "From hundreds to thousands: widening the normal human Urinome". The material supplied to Chorus Progect.org includes technical MS spectra data only.
AB - The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name "From hundreds to thousands: widening the normal human Urinome". The material supplied to Chorus Progect.org includes technical MS spectra data only.
KW - Combinatorial peptide ligand libraries
KW - Mass spectrometry
KW - Urinary proteome
KW - Vesicles
UR - http://www.scopus.com/inward/record.url?scp=84923653148&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923653148&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2014.08.006
DO - 10.1016/j.dib.2014.08.006
M3 - Article
AN - SCOPUS:84923653148
SN - 2352-3409
VL - 1
SP - 25
EP - 28
JO - Data in Brief
JF - Data in Brief
ER -