Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

George Adrian Calin; Calin Dan Dumitru; Masayoshi Shimizu; Roberta Bichi; Simona Zupo; Evan Noch; Hansjuerg Aldler; Sashi Rattan; Michael Keating; Kanti Rai; Laura Rassenti; Thomas Kipps; Massimo Negrini; Florencia Bullrich; Carlo M. Croce

doi:10.1073/pnas.242606799

Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

George Adrian Calin, Calin Dan Dumitru, Masayoshi Shimizu, Roberta Bichi, Simona Zupo, Evan Noch, Hansjuerg Aldler, Sashi Rattan, Michael Keating, Kanti Rai, Laura Rassenti, Thomas Kipps, Massimo Negrini, Florencia Bullrich, Carlo M. Croce

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors (≈70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority (≈68%) of CLL cases.

Original language	English
Pages (from-to)	15524-15529
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	24
DOIs	https://doi.org/10.1073/pnas.242606799
Publication status	Published - Nov 26 2002

ASJC Scopus subject areas

Genetics
General

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Calin, G. A., Dumitru, C. D., Shimizu, M., Bichi, R., Zupo, S., Noch, E., Aldler, H., Rattan, S., Keating, M., Rai, K., Rassenti, L., Kipps, T., Negrini, M., Bullrich, F., & Croce, C. M. (2002). Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proceedings of the National Academy of Sciences of the United States of America, 99(24), 15524-15529. https://doi.org/10.1073/pnas.242606799

Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. / Calin, George Adrian; Dumitru, Calin Dan; Shimizu, Masayoshi et al.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 24, 26.11.2002, p. 15524-15529.

Research output: Contribution to journal › Article › peer-review

Calin, GA, Dumitru, CD, Shimizu, M, Bichi, R, Zupo, S, Noch, E, Aldler, H, Rattan, S, Keating, M, Rai, K, Rassenti, L, Kipps, T, Negrini, M, Bullrich, F & Croce, CM 2002, 'Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 24, pp. 15524-15529. https://doi.org/10.1073/pnas.242606799

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abstract = "Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors (≈70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority (≈68%) of CLL cases.",

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T1 - Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

AU - Calin, George Adrian

AU - Dumitru, Calin Dan

AU - Shimizu, Masayoshi

AU - Bichi, Roberta

AU - Zupo, Simona

AU - Noch, Evan

AU - Aldler, Hansjuerg

AU - Rattan, Sashi

AU - Keating, Michael

AU - Rai, Kanti

AU - Rassenti, Laura

AU - Kipps, Thomas

AU - Negrini, Massimo

AU - Bullrich, Florencia

AU - Croce, Carlo M.

PY - 2002/11/26

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AB - Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors (≈70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority (≈68%) of CLL cases.

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Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

Abstract

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