TY - JOUR
T1 - Flow cytometry applications for the analysis of chemokine receptor expression and function
AU - Anselmo, Achille
AU - Mazzon, Cristina
AU - Borroni, Elena Monica
AU - Bonecchi, Raffaella
AU - Graham, Gerard J.
AU - Locati, Massimo
PY - 2014
Y1 - 2014
N2 - Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities.
AB - Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities.
KW - Antibodies
KW - Chemokine receptor
KW - Flow cytometry
KW - Fluorophores
UR - http://www.scopus.com/inward/record.url?scp=84897116174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897116174&partnerID=8YFLogxK
U2 - 10.1002/cyto.a.22439
DO - 10.1002/cyto.a.22439
M3 - Article
C2 - 24464630
AN - SCOPUS:84897116174
SN - 1552-4922
VL - 85
SP - 292
EP - 301
JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology
JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology
IS - 4
ER -