Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl- current and calcium mobilization in human tracheal 9HTEo- cells

Luis J V Galietta; Andrea Rasola; Michela Rugolo; Michela Zottini; Teresa Mastrocola; Dieter C. Gruenert; Giovanni Romeo

doi:10.1016/0014-5793(92)80589-9

Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl- current and calcium mobilization in human tracheal 9HTEo- cells

Luis J V Galietta, Andrea Rasola, Michela Rugolo, Michela Zottini, Teresa Mastrocola, Dieter C. Gruenert, Giovanni Romeo

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo-) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01-100 μM) caused activation of Cl^- currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca²⁺ or by the addition of the A₂ adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca²⁺ influx through the plasma membrane and a release from intracellular stores.

Original language	English
Pages (from-to)	61-65
Number of pages	5
Journal	FEBS Letters
Volume	304
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(92)80589-9
Publication status	Published - Jun 8 1992

Keywords

2-Chloroadenosine
Airway epithelium
ATP
Cell volume
Cl current
Intracellular Ca

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(92)80589-9

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title = "Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl- current and calcium mobilization in human tracheal 9HTEo- cells",

abstract = "The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo-) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01-100 μM) caused activation of Cl- currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca2+ or by the addition of the A2 adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca2+ influx through the plasma membrane and a release from intracellular stores.",

keywords = "2-Chloroadenosine, Airway epithelium, ATP, Cell volume, Cl current, Intracellular Ca",

author = "Galietta, {Luis J V} and Andrea Rasola and Michela Rugolo and Michela Zottini and Teresa Mastrocola and Gruenert, {Dieter C.} and Giovanni Romeo",

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T1 - Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl- current and calcium mobilization in human tracheal 9HTEo- cells

AU - Galietta, Luis J V

AU - Rasola, Andrea

AU - Rugolo, Michela

AU - Zottini, Michela

AU - Mastrocola, Teresa

AU - Gruenert, Dieter C.

AU - Romeo, Giovanni

PY - 1992/6/8

Y1 - 1992/6/8

N2 - The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo-) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01-100 μM) caused activation of Cl- currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca2+ or by the addition of the A2 adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca2+ influx through the plasma membrane and a release from intracellular stores.

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KW - 2-Chloroadenosine

KW - Airway epithelium

KW - ATP

KW - Cell volume

KW - Cl current

KW - Intracellular Ca

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Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl- current and calcium mobilization in human tracheal 9HTEo- cells

Abstract

Keywords

ASJC Scopus subject areas

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