Expression of high- and low-affinity epidermal growth factor receptors in human hepatoma cell lines

Massimo Clementi; Antonella Festa; Ivano Testa; Patrizia Bagnarelli; Giulia Devescovi; Guido Carloni

doi:10.1016/0014-5793(89)80645-3

Expression of high- and low-affinity epidermal growth factor receptors in human hepatoma cell lines

Massimo Clementi, Antonella Festa, Ivano Testa, Patrizia Bagnarelli, Giulia Devescovi, Guido Carloni

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum- and hormone-free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.

Original language	English
Pages (from-to)	297-301
Number of pages	5
Journal	FEBS Letters
Volume	249
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)80645-3
Publication status	Published - Jun 5 1989

Keywords

(Human hepatoma cell)
Cell replication
Epidermal growth factor receptor

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(89)80645-3

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title = "Expression of high- and low-affinity epidermal growth factor receptors in human hepatoma cell lines",

abstract = "Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum- and hormone-free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.",

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T1 - Expression of high- and low-affinity epidermal growth factor receptors in human hepatoma cell lines

AU - Clementi, Massimo

AU - Festa, Antonella

AU - Testa, Ivano

AU - Bagnarelli, Patrizia

AU - Devescovi, Giulia

AU - Carloni, Guido

PY - 1989/6/5

Y1 - 1989/6/5

N2 - Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum- and hormone-free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.

AB - Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum- and hormone-free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.

KW - (Human hepatoma cell)

KW - Cell replication

KW - Epidermal growth factor receptor

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Expression of high- and low-affinity epidermal growth factor receptors in human hepatoma cell lines

Abstract

Keywords

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