Expression of CD20 reveals a new store-operated calcium entry modulator in skeletal muscle

Daniele Parolini, Letizia Cassinelli, Paola Razini, Clementina Sitzia, Noemi Tonna, Silvia Erratico, Federica Colleoni, Valentina Angeloni, Elisa Maffioli, Andrea Farini, Simona MacIotta, Laura Porretti, Marzia Belicchi, Fabio Bianco, Gabriella Tedeschi, Mirella Meregalli, Yvan Torrente

Research output: Contribution to journalArticlepeer-review


Among the scarce available data about the biological role of the membrane protein CD20, there is some evidence that this protein functions as a store-operated Ca2+ channel and/or regulates transmembrane Ca 2+ trafficking. Recent findings indicate that store-operated Ca 2+ entry (SOCE) plays a central role in skeletal muscle function and development, but there remain a number of unresolved issues relating to SOCE modulation in this tissue. Here we describe CD20 expression in skeletal muscle, verifying its membrane localization in myoblasts and adult muscle fibers. Additionally, we show that inhibition of CD20 through antibody binding or gene silencing resulted in specific impairment of SOCE in C2C12 myoblasts. Our results provide novel insights into the CD20 expression pattern, and suggest that functional CD20 is required for SOCE to consistently occur in C2C12 myoblasts. These findings may contribute to future identification of mechanisms and molecules involved in the fine regulation of store-operated Ca2+ entry in skeletal muscle.

Original languageEnglish
Pages (from-to)2095-2105
Number of pages11
JournalInternational Journal of Biochemistry and Cell Biology
Issue number12
Publication statusPublished - Dec 2012


  • Ca
  • CD20
  • Skeletal muscle
  • SOCE

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology


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