TY - JOUR
T1 - Expression of amyloid precursor protein isoforms in neuroectodermal cell lines upon retinoic acid exposure
AU - Erba, F.
AU - Candi, E.
AU - Annicchiarico-Petruzzelli, M.
AU - Pagella, P.
AU - Brufani, M.
AU - Melino, G.
AU - Ascoli, F.
PY - 1993
Y1 - 1993
N2 - Different neuroectodermal cell lines differentiated by retinoic acid express various protein isoforms of the amyloid precursor protein (APP). Human neuroblastoma [SK-N-BE(2), SK-N-SH, SH-SY5Y, CHP 100, SMS-KNR], and melanoma [SK-Mel 28, TVM-BO] cell lines produced two major APP of about 103 and 93 kDa. The glioblastoma line [LI] expresses only the larger APP isoform. With one exception, all cell lines showed only minor augmentation in intensity and ratio between the two isoforms after 5 days exposure to 5 μM retinoic acid. However, SH-SY5Y cells showed the induction of a smaller APP isoform of about 81 kDa accompanied by a relative reduction in intensity of the two larger proteins. These data were also confirmed by immunohistochemistry, which also showed no changes in intracellular localisation of APP, but with considerable cell-to-cell heterogeneity suggesting heterogeneous cell regulation.
AB - Different neuroectodermal cell lines differentiated by retinoic acid express various protein isoforms of the amyloid precursor protein (APP). Human neuroblastoma [SK-N-BE(2), SK-N-SH, SH-SY5Y, CHP 100, SMS-KNR], and melanoma [SK-Mel 28, TVM-BO] cell lines produced two major APP of about 103 and 93 kDa. The glioblastoma line [LI] expresses only the larger APP isoform. With one exception, all cell lines showed only minor augmentation in intensity and ratio between the two isoforms after 5 days exposure to 5 μM retinoic acid. However, SH-SY5Y cells showed the induction of a smaller APP isoform of about 81 kDa accompanied by a relative reduction in intensity of the two larger proteins. These data were also confirmed by immunohistochemistry, which also showed no changes in intracellular localisation of APP, but with considerable cell-to-cell heterogeneity suggesting heterogeneous cell regulation.
KW - βA4
KW - Alzheimer
KW - APP
KW - Neuroblastoma
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M3 - Article
AN - SCOPUS:0027724602
SN - 0892-2187
VL - 6
SP - 55
EP - 61
JO - Clinical Chemistry and Enzymology Communications
JF - Clinical Chemistry and Enzymology Communications
IS - 1-2
ER -