Expression in lung tumors and genetic mapping of the novel murine protein kinase Cη

F. Canzian; M. Gariboldi; G. Manenti; L. De Gregorio; S. I. Osada; S. Ohno; T. A. Dragani; M. A. Pierotti

doi:10.1002/mc.2940090302

Expression in lung tumors and genetic mapping of the novel murine protein kinase Cη

F. Canzian, M. Gariboldi, G. Manenti, L. De Gregorio, S. I. Osada, S. Ohno, T. A. Dragani, M. A. Pierotti

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Protein kinase Cη (nPKCη) is a member of the protein kinase C family with a unique tissue distribution in skin and lung. We analyzed nPKCη expression in normal murine lung and in 36 lung tumors induced by urethane in AC3 F1 and F2 mice. The nPKCη-related transcript was present at fivefold to tenfold lower levels in tumors than in normal lung controls. We mapped two distinct loci for nPKCη on murine chromosome 12, using linkage analysis in an interspecific test cross. The results indicate that the mouse Pkcn-rs1 and Pkcn-rs2 loci were about 30 cM from the centromere and about 2 cM from each other.

Original language	English
Pages (from-to)	111-113
Number of pages	3
Journal	Molecular Carcinogenesis
Volume	9
Issue number	3
DOIs	https://doi.org/10.1002/mc.2940090302
Publication status	Published - 1994

Keywords

Chromosome mapping
gene expression
genetic linkage
genetic polymorphism
restriction fragment length polymorphism

ASJC Scopus subject areas

Cancer Research
Molecular Biology

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10.1002/mc.2940090302

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title = "Expression in lung tumors and genetic mapping of the novel murine protein kinase Cη",

abstract = "Protein kinase Cη (nPKCη) is a member of the protein kinase C family with a unique tissue distribution in skin and lung. We analyzed nPKCη expression in normal murine lung and in 36 lung tumors induced by urethane in AC3 F1 and F2 mice. The nPKCη-related transcript was present at fivefold to tenfold lower levels in tumors than in normal lung controls. We mapped two distinct loci for nPKCη on murine chromosome 12, using linkage analysis in an interspecific test cross. The results indicate that the mouse Pkcn-rs1 and Pkcn-rs2 loci were about 30 cM from the centromere and about 2 cM from each other.",

keywords = "Chromosome mapping, gene expression, genetic linkage, genetic polymorphism, restriction fragment length polymorphism",

author = "F. Canzian and M. Gariboldi and G. Manenti and {De Gregorio}, L. and Osada, {S. I.} and S. Ohno and Dragani, {T. A.} and Pierotti, {M. A.}",

year = "1994",

doi = "10.1002/mc.2940090302",

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T1 - Expression in lung tumors and genetic mapping of the novel murine protein kinase Cη

AU - Canzian, F.

AU - Gariboldi, M.

AU - Manenti, G.

AU - De Gregorio, L.

AU - Osada, S. I.

AU - Ohno, S.

AU - Dragani, T. A.

AU - Pierotti, M. A.

PY - 1994

Y1 - 1994

N2 - Protein kinase Cη (nPKCη) is a member of the protein kinase C family with a unique tissue distribution in skin and lung. We analyzed nPKCη expression in normal murine lung and in 36 lung tumors induced by urethane in AC3 F1 and F2 mice. The nPKCη-related transcript was present at fivefold to tenfold lower levels in tumors than in normal lung controls. We mapped two distinct loci for nPKCη on murine chromosome 12, using linkage analysis in an interspecific test cross. The results indicate that the mouse Pkcn-rs1 and Pkcn-rs2 loci were about 30 cM from the centromere and about 2 cM from each other.

AB - Protein kinase Cη (nPKCη) is a member of the protein kinase C family with a unique tissue distribution in skin and lung. We analyzed nPKCη expression in normal murine lung and in 36 lung tumors induced by urethane in AC3 F1 and F2 mice. The nPKCη-related transcript was present at fivefold to tenfold lower levels in tumors than in normal lung controls. We mapped two distinct loci for nPKCη on murine chromosome 12, using linkage analysis in an interspecific test cross. The results indicate that the mouse Pkcn-rs1 and Pkcn-rs2 loci were about 30 cM from the centromere and about 2 cM from each other.

KW - Chromosome mapping

KW - gene expression

KW - genetic linkage

KW - genetic polymorphism

KW - restriction fragment length polymorphism

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Expression in lung tumors and genetic mapping of the novel murine protein kinase Cη

Abstract

Keywords

ASJC Scopus subject areas

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