Expression and functions of the vascular endothelial growth factors and their receptors in human basophils

Amato De Paulis, Nella Prevete, Isabella Fiorentino, Francesca Wanda Rossi, Stefania Staibano, Nunzia Montuori, Pia Ragno, Amelia Longobardi, Bianca Liccardo, Arturo Genovese, Domenico Ribatti, Andrew F. Walls, Gianni Marone

Research output: Contribution to journalArticlepeer-review


Angiogenesis is a multistep complex phenomenon critical for several inflammatory and neoplastic disorders. Basophils, normally-confined to peripheral blood, can infiltrate the sites of chronic inflammation. In an attempt to obtain insights into the mechanism(s) underlying human basophil chemotaxis and its role in inflammation, we have characterized the expression and function of vascular endothelial growth factors (VEGFs) and their receptors in these cells. Basophils express mRNA for three isoforms of VEGF-A (121, 165, and 189) and two isoforms of VEGF-B (167 and 186). Peripheral blood and basophils in nasal polyps contain VEGF-A localized in secretory granules. The concentration of VEGF-A in basophils was 144.4 ± 10.8 pg/106 cells. Immunologic activation of basophils induced the release of VEGF-A. VEGF-A (10-500 ng/ml) induced basophil chemotaxis. Supernatants of activated basophils induced an angiogenic response in the chick embryo chorioallantoic membrane that was inhibited by an anti-VEGF-A Ab. The tyrosine kinase VEGFR-2 (VEGFR-2/KDR) mRNA was expressed in basophils. These cells also expressed mRNA for the soluble form of VEGFR-1 and neuropilin (NRP)1 and NRP2. Flow cytometric analysis indicated that basophils express epitopes recognized by mAbs against the extracellular domains of VEGFR-2, NRP1, and NRP2. Our data suggest that basophils could play a role in angiogenesis and inflammation through the expression of several forms of VEGF and their receptors.

Original languageEnglish
Pages (from-to)7322-7331
Number of pages10
JournalJournal of Immunology
Issue number10
Publication statusPublished - Nov 15 2006

ASJC Scopus subject areas

  • Immunology


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