Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

R. Zamarchi; P. Allavena; A. Borsetti; L. Stievano; V. Tosello; N. Marcato; G. Esposito; V. Roni; C. Paganin; G. Bianchi; F. Titti; P. Verani; G. Gerosa; A. Amadori

doi:10.1046/j.1365-2249.2002.01775.x

Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

R. Zamarchi, P. Allavena, A. Borsetti, L. Stievano, V. Tosello, N. Marcato, G. Esposito, V. Roni, C. Paganin, G. Bianchi, F. Titti, P. Verani, G. Gerosa, A. Amadori

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Abstract

In this paper we addressed the expression of the HIV co-receptors CXCR-4 and CCR-5 in human thymocytes by phenotypic, molecular and functional approaches. Cytofluorimetric analysis disclosed that CXCR-4 was constitutively expressed by freshly isolated thymocytes (∼10 000 molecules/cell in about 30% of thymocytes); the receptor was endowed with functional activity, as it mediated polarization, migration and intracellular Ca ²⁺ increase in response to its ligand, SDF-1. On the contrary, CCR-5 expression in freshly isolated thymocytes was significantly lower (

Original language	English
Pages (from-to)	321-330
Number of pages	10
Journal	Clinical and Experimental Immunology
Volume	127
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2249.2002.01775.x
Publication status	Published - 2002

Keywords

CCR-5
CXCR-4
HIV
Thymus

ASJC Scopus subject areas

Immunology

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10.1046/j.1365-2249.2002.01775.x

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Zamarchi, R., Allavena, P., Borsetti, A., Stievano, L., Tosello, V., Marcato, N., Esposito, G., Roni, V., Paganin, C., Bianchi, G., Titti, F., Verani, P., Gerosa, G., & Amadori, A. (2002). Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes. Clinical and Experimental Immunology, 127(2), 321-330. https://doi.org/10.1046/j.1365-2249.2002.01775.x

Zamarchi, R , Allavena, P, Borsetti, A, Stievano, L, Tosello, V, Marcato, N, Esposito, G, Roni, V, Paganin, C, Bianchi, G, Titti, F, Verani, P, Gerosa, G & Amadori, A 2002, 'Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes', Clinical and Experimental Immunology, vol. 127, no. 2, pp. 321-330. https://doi.org/10.1046/j.1365-2249.2002.01775.x

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AU - Zamarchi, R.

AU - Allavena, P.

AU - Borsetti, A.

AU - Stievano, L.

AU - Tosello, V.

AU - Marcato, N.

AU - Esposito, G.

AU - Roni, V.

AU - Paganin, C.

AU - Bianchi, G.

AU - Titti, F.

AU - Verani, P.

AU - Gerosa, G.

AU - Amadori, A.

PY - 2002

Y1 - 2002

N2 - In this paper we addressed the expression of the HIV co-receptors CXCR-4 and CCR-5 in human thymocytes by phenotypic, molecular and functional approaches. Cytofluorimetric analysis disclosed that CXCR-4 was constitutively expressed by freshly isolated thymocytes (∼10 000 molecules/cell in about 30% of thymocytes); the receptor was endowed with functional activity, as it mediated polarization, migration and intracellular Ca 2+ increase in response to its ligand, SDF-1. On the contrary, CCR-5 expression in freshly isolated thymocytes was significantly lower (

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KW - CCR-5

KW - CXCR-4

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Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

Abstract

Keywords

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