TY - JOUR
T1 - Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
AU - Gualandi, Chiara
AU - Govoni, Marco
AU - Foroni, Laura
AU - Valente, Sabrina
AU - Bianchi, Michele
AU - Giordano, Emanuele
AU - Pasquinelli, Gianandrea
AU - Biscarini, Fabio
AU - Focarete, Maria Letizia
PY - 2012/12
Y1 - 2012/12
N2 - Disinfection of electrospun materials is commonly carried out by ethanol (EtOH) soaking. However, in the literature no systematic investigation on the effect of EtOH disinfection on scaffold properties is available. In this study electrospun poly(l-lactic acid) (PLLA) scaffolds were differently treated in EtOH and changes of fiber morphology, fiber surface topography and scaffold thermo-mechanical properties were evaluated. It was found that EtOH induced the formation of an amount of crystal phase, in the initially amorphous fibers, which depends on EtOH grade, soaking time and temperature. Completely amorphous PLLA (a-PLLA) and semicrystalline PLLA (sc-PLLA) scaffolds were produced by applying different EtOH treatments to as-spun PLLA scaffold. Compared to a-PLLA, sc-PLLA was stiffer and composed of fibers with rougher surface. Cell culture performed by using permanent cell line H9c2 demonstrated that changes of scaffold properties determined a different cell response, in particular in the expression of proteins correlated to cell motility and cell adhesion.
AB - Disinfection of electrospun materials is commonly carried out by ethanol (EtOH) soaking. However, in the literature no systematic investigation on the effect of EtOH disinfection on scaffold properties is available. In this study electrospun poly(l-lactic acid) (PLLA) scaffolds were differently treated in EtOH and changes of fiber morphology, fiber surface topography and scaffold thermo-mechanical properties were evaluated. It was found that EtOH induced the formation of an amount of crystal phase, in the initially amorphous fibers, which depends on EtOH grade, soaking time and temperature. Completely amorphous PLLA (a-PLLA) and semicrystalline PLLA (sc-PLLA) scaffolds were produced by applying different EtOH treatments to as-spun PLLA scaffold. Compared to a-PLLA, sc-PLLA was stiffer and composed of fibers with rougher surface. Cell culture performed by using permanent cell line H9c2 demonstrated that changes of scaffold properties determined a different cell response, in particular in the expression of proteins correlated to cell motility and cell adhesion.
KW - Crystallinity
KW - Electrospinning
KW - H9c2 cells
KW - Polylactic acid
KW - Sterilization
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U2 - 10.1016/j.eurpolymj.2012.09.016
DO - 10.1016/j.eurpolymj.2012.09.016
M3 - Article
AN - SCOPUS:84868205826
SN - 0014-3057
VL - 48
SP - 2008
EP - 2018
JO - European Polymer Journal
JF - European Polymer Journal
IS - 12
ER -