TY - JOUR
T1 - Enhanced monocyte expression of tissue factor by oxidative stress in patients with antiphospholipid antibodies
T2 - Effect of antioxidant treatment
AU - Ferro, D.
AU - Saliola, M.
AU - Meroni, P. L.
AU - Valesini, G.
AU - Caroselli, C.
AU - Praticó, D.
AU - Fitzgerald, G. A.
AU - Shoenfeld, Y.
AU - Violi, F.
PY - 2003/3
Y1 - 2003/3
N2 - In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.
AB - In a first study, we performed a cross-sectional analysis of urinary excretion of isoprostanes, IPF 2α-III and VI, and monocyte tissue factor (TF) antigen and activity between 11 antiphospholipid (APL) antibody-positive patients and 13 APL negative subjects. In a second study, 11 APL positive patients were randomly supplemented either with (n=6) or without (n=5) antioxidants (vitamin E at 900 IU day -1, vitamin C at 2000 mg day -1) for 6 weeks. In a third study, TF and superoxide anion were measured in human monocytes incubated with anti β 2 glycoprotein 1 (β 2GP 1) or control IgG, either with or without vitamin E. APL-positive patients had higher values of isoprostanes (P2α-III (rho 0.79; P2α-VI (rho=0.87; P2GP 1 antibodies dose-dependently enhanced the monocyte production of the superoxide anion and TF, which were significantly inhibited by vitamin E. This study demonstrates that in APL-positive patients, oxidative stress contributes to activate the clotting system via over-expression of monocyte TF. We suggest that anti-β 2GP 1 antibodies could play a pivotal role by enhancing the monocyte production of oxygen free radicals.
KW - Antiphospholipid antibodies
KW - Oxidative stress
KW - Tissue factor
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U2 - 10.1046/j.1538-7836.2003.00108.x
DO - 10.1046/j.1538-7836.2003.00108.x
M3 - Article
C2 - 12871461
AN - SCOPUS:0642280708
SN - 1538-7933
VL - 1
SP - 523
EP - 531
JO - Journal of Thrombosis and Haemostasis
JF - Journal of Thrombosis and Haemostasis
IS - 3
ER -