Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblasts

Donatella Granchi, Susanna Stea, Gabriela Ciapetti, Daniela Cavedagna, Stefano Stea, Arturo Pizzoferrato

Research output: Contribution to journalArticlepeer-review


The effects of endodontic cements on the cell cycle of MG63 osteoblasts cultured in vitro have been examined. Three groups of compounds were tested. Group I encompassed zinc oxide- and eugenol-based cements (Tubliseal, Argoseal, N2), group II consisted of cements with a phenol group other than eugenol (AH26, Forfenan, Methode R/R), and group III included CaOH-based cements (Biocalex, Endocalex). The cell cycle of MG63 cells was analyzed by flow cytometry; the DNA content was evaluated by means of the propidium iodide uptake method, whereas the proportion of cells in the S phase was defined by the incorporation of bromodeoxyuridine later revealed by a specific antibody. The results showed that some root canal sealers could hamper the periapex healing processes by inhibiting the cell proliferation through a selective action on different phases of the cell cycle.

Original languageEnglish
Pages (from-to)359-366
Number of pages8
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Issue number3
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Dentistry(all)
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Radiology Nuclear Medicine and imaging


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