EMT markers in lung adenocarcinoma pleural effusion spheroid cells

Enrico Giarnieri; Claudia De Vitis; Alessia Noto; Giuseppe Roscilli; Gerardo Salerno; Salvatore Mariotta; Alberto Ricci; Pierdonato Bruno; Giuseppe Russo; Andrea Laurenzi; Maria Rosaria Giovagnoli; Gennaro Ciliberto; Rita Mancini

doi:10.1002/jcp.24300

EMT markers in lung adenocarcinoma pleural effusion spheroid cells

Enrico Giarnieri, Claudia De Vitis, Alessia Noto, Giuseppe Roscilli, Gerardo Salerno, Salvatore Mariotta, Alberto Ricci, Pierdonato Bruno, Giuseppe Russo, Andrea Laurenzi, Maria Rosaria Giovagnoli, Gennaro Ciliberto, Rita Mancini

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article › peer-review

Abstract

Epithelial-to-mesenchymal transition (EMT) is a process in which cells undergo a developmental switch from epithelial to mesenchymal phenotype. This process has been related to embryologic morphogenesis but also to cancer progression and metastasis. The aim of the current study was to investigate the expression of EMT-related markers in adherent and spheroid cell cultures derived from malignant pleural effusions (MPEs) of patients affected by lung adenocarcinoma. On the basis of efficient in vitro propagation, six cases of MPEs were selected and analyzed by immunocytochemistry staining for EMT markers and by RT-PCR for transcription factors known to orchestrate EMT. EMT markers immunostaining showed in spheroids a statistically significant correlation between the loss of E-cadherin immunoreactivity and overexpression of N-cadherin (P

Original language	English
Pages (from-to)	1720-1726
Number of pages	7
Journal	Journal of Cellular Physiology
Volume	228
Issue number	8
DOIs	https://doi.org/10.1002/jcp.24300
Publication status	Published - Aug 2013

ASJC Scopus subject areas

Clinical Biochemistry
Cell Biology
Physiology

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10.1002/jcp.24300

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title = "EMT markers in lung adenocarcinoma pleural effusion spheroid cells",

abstract = "Epithelial-to-mesenchymal transition (EMT) is a process in which cells undergo a developmental switch from epithelial to mesenchymal phenotype. This process has been related to embryologic morphogenesis but also to cancer progression and metastasis. The aim of the current study was to investigate the expression of EMT-related markers in adherent and spheroid cell cultures derived from malignant pleural effusions (MPEs) of patients affected by lung adenocarcinoma. On the basis of efficient in vitro propagation, six cases of MPEs were selected and analyzed by immunocytochemistry staining for EMT markers and by RT-PCR for transcription factors known to orchestrate EMT. EMT markers immunostaining showed in spheroids a statistically significant correlation between the loss of E-cadherin immunoreactivity and overexpression of N-cadherin (P",

author = "Enrico Giarnieri and {De Vitis}, Claudia and Alessia Noto and Giuseppe Roscilli and Gerardo Salerno and Salvatore Mariotta and Alberto Ricci and Pierdonato Bruno and Giuseppe Russo and Andrea Laurenzi and Giovagnoli, {Maria Rosaria} and Gennaro Ciliberto and Rita Mancini",

year = "2013",

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AU - Giarnieri, Enrico

AU - De Vitis, Claudia

AU - Noto, Alessia

AU - Roscilli, Giuseppe

AU - Salerno, Gerardo

AU - Mariotta, Salvatore

AU - Ricci, Alberto

AU - Bruno, Pierdonato

AU - Russo, Giuseppe

AU - Laurenzi, Andrea

AU - Giovagnoli, Maria Rosaria

AU - Ciliberto, Gennaro

AU - Mancini, Rita

PY - 2013/8

Y1 - 2013/8

N2 - Epithelial-to-mesenchymal transition (EMT) is a process in which cells undergo a developmental switch from epithelial to mesenchymal phenotype. This process has been related to embryologic morphogenesis but also to cancer progression and metastasis. The aim of the current study was to investigate the expression of EMT-related markers in adherent and spheroid cell cultures derived from malignant pleural effusions (MPEs) of patients affected by lung adenocarcinoma. On the basis of efficient in vitro propagation, six cases of MPEs were selected and analyzed by immunocytochemistry staining for EMT markers and by RT-PCR for transcription factors known to orchestrate EMT. EMT markers immunostaining showed in spheroids a statistically significant correlation between the loss of E-cadherin immunoreactivity and overexpression of N-cadherin (P

AB - Epithelial-to-mesenchymal transition (EMT) is a process in which cells undergo a developmental switch from epithelial to mesenchymal phenotype. This process has been related to embryologic morphogenesis but also to cancer progression and metastasis. The aim of the current study was to investigate the expression of EMT-related markers in adherent and spheroid cell cultures derived from malignant pleural effusions (MPEs) of patients affected by lung adenocarcinoma. On the basis of efficient in vitro propagation, six cases of MPEs were selected and analyzed by immunocytochemistry staining for EMT markers and by RT-PCR for transcription factors known to orchestrate EMT. EMT markers immunostaining showed in spheroids a statistically significant correlation between the loss of E-cadherin immunoreactivity and overexpression of N-cadherin (P

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EMT markers in lung adenocarcinoma pleural effusion spheroid cells

Abstract

ASJC Scopus subject areas

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