TY - JOUR
T1 - Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40
AU - Guerrasio, Angelo
AU - Saglio, Giuseppe
AU - Mazza, Umberto
AU - Pich, Piergiorgio
AU - Camaschella, Clara
AU - Ricco, Giuseppe
AU - Gianazza, Elisabetta
AU - Righetti, Pier Giorgio
PY - 1979/11/15
Y1 - 1979/11/15
N2 - Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, Aγ and Gγ human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment 7CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the Aγ chain, thus masking either a Lys or an Arg residue at the two extremes.
AB - Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, Aγ and Gγ human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment 7CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the Aγ chain, thus masking either a Lys or an Arg residue at the two extremes.
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U2 - 10.1016/0009-8981(79)90133-5
DO - 10.1016/0009-8981(79)90133-5
M3 - Article
C2 - 498543
AN - SCOPUS:0018596053
SN - 0009-8981
VL - 99
SP - 7
EP - 11
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1
ER -