Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy

Miguel Otero; Sandra A. Calarota; Anlan Dai; Anne S. De Groot; Jean D. Boyer; David B. Weiner

doi:10.1016/j.vaccine.2005.08.010

Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy

Miguel Otero, Sandra A. Calarota, Anlan Dai, Anne S. De Groot, Jean D. Boyer, David B. Weiner

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

We tested a DNA vaccine strategy in order to improve the efficacy and safety of the current live smallpox vaccine involving priming with DNA vaccines and boosting with live vaccinia virus (VacV). We generated DNA plasmids encoding the A4L, A27L and H5R VacV genes. A considerable increase in antigen-specific IFN-γ responses, high proliferative and humoral antigen-specific responses were detected in experimental primed Balb/C mice compared to controls after VacV boost. The VacV-DNA plasmids elicited IFN-γ production in HLA-A2.1 transgenic mice in response to predicted HLA-A2.1 restricted peptide epitopes, providing valuable data for further vaccine development.

Original language	English
Pages (from-to)	4461-4470
Number of pages	10
Journal	Vaccine
Volume	24
Issue number	21
DOIs	https://doi.org/10.1016/j.vaccine.2005.08.010
Publication status	Published - May 22 2006

Keywords

Cellular and humoral immune responses
DNA vaccines
Smallpox

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

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10.1016/j.vaccine.2005.08.010

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title = "Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy",

abstract = "We tested a DNA vaccine strategy in order to improve the efficacy and safety of the current live smallpox vaccine involving priming with DNA vaccines and boosting with live vaccinia virus (VacV). We generated DNA plasmids encoding the A4L, A27L and H5R VacV genes. A considerable increase in antigen-specific IFN-γ responses, high proliferative and humoral antigen-specific responses were detected in experimental primed Balb/C mice compared to controls after VacV boost. The VacV-DNA plasmids elicited IFN-γ production in HLA-A2.1 transgenic mice in response to predicted HLA-A2.1 restricted peptide epitopes, providing valuable data for further vaccine development.",

keywords = "Cellular and humoral immune responses, DNA vaccines, Smallpox",

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T1 - Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy

AU - Otero, Miguel

AU - Calarota, Sandra A.

AU - Dai, Anlan

AU - De Groot, Anne S.

AU - Boyer, Jean D.

AU - Weiner, David B.

PY - 2006/5/22

Y1 - 2006/5/22

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AB - We tested a DNA vaccine strategy in order to improve the efficacy and safety of the current live smallpox vaccine involving priming with DNA vaccines and boosting with live vaccinia virus (VacV). We generated DNA plasmids encoding the A4L, A27L and H5R VacV genes. A considerable increase in antigen-specific IFN-γ responses, high proliferative and humoral antigen-specific responses were detected in experimental primed Balb/C mice compared to controls after VacV boost. The VacV-DNA plasmids elicited IFN-γ production in HLA-A2.1 transgenic mice in response to predicted HLA-A2.1 restricted peptide epitopes, providing valuable data for further vaccine development.

KW - Cellular and humoral immune responses

KW - DNA vaccines

KW - Smallpox

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Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy

Abstract

Keywords

ASJC Scopus subject areas

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