Effect of recombinant human TNF on human ovarian cancer cell lines.

A. Vigani, L. Miglietta, S. Chiara, L. Repetto, E. Pichi, G. Cimoli, L. Morelli, L. Poggi, G. Billi, S. Parodi

Research output: Contribution to journalArticlepeer-review


Ovarian cancer is the second most common gynecologic malignancy. Standard therapeutic approaches to this disease, surgery followed by chemotherapy, have produced response rates of up to 80%. However, the five-year survival rate remains around 30%. Recently, Tumor Necrosis Factor (TNF) has received attention as either an alternative or an associated agent for chemotherapy of ovarian cancer. TNF is known to have direct cytotoxic and cytostatic effects on a variety of transformed cell lines "in vitro". Furthermore, TNF is known to enhance significantly the "in vitro" effects of a class of chemotherapeutic agents, specifically those targeted at DNA topoisomerase II. In this work we have investigated TNF-induced cytotoxicity in four established human epithelial ovarian cancer cell lines: A-2774; SV-626; SKOV-3 and Pa-1. TNF mediated cytotoxic activity was observed in a range of concentrations between 1 U/ml and 10-3 U/ml. A-2774 and SV-626 were the two most sensitive lines, especially when exposed to high concentrations of TNF.

Original languageEnglish
Pages (from-to)537-542
Number of pages6
JournalBollettino della Societa Italiana di Biologia Sperimentale
Issue number6
Publication statusPublished - Jun 1990

ASJC Scopus subject areas

  • Medicine(all)


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