TY - JOUR
T1 - Effect of four acrylic bone cements on transforming growth factor-beta1 expression by osteoblast-like cells MG63
AU - Cenni, Elisabetta
AU - Granchi, Donatella
AU - Ciapetti, Gabriela
AU - Savarino, Lucia
AU - Corradini, Alessandra
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-β1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, following 1h- and 7 day-curing. MG63 cells seldom expressed mRNA specific for TGF-β1 in basal conditions. The cultures expressed mRNA constantly after incubation with the extract of CMW 1 cured for 1h. TGF-β1 specific mRNA was seldom expressed after incubation with the other cement extracts. The release of TGF-β1 into the conditioned medium was increased significantly by CMW 1 extract at 1h-curing, but was not changed significantly by CMW 1 extract at 7 day-curing and by the extracts of the other cements, at both curing times. The stimulating effect of CMW 1 on the secretion of TGF-β1, even with all the restrictions of an in vitro study of continuous cell lines, if confirmed in vivo, might favor the development of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis.
AB - Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-β1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, following 1h- and 7 day-curing. MG63 cells seldom expressed mRNA specific for TGF-β1 in basal conditions. The cultures expressed mRNA constantly after incubation with the extract of CMW 1 cured for 1h. TGF-β1 specific mRNA was seldom expressed after incubation with the other cement extracts. The release of TGF-β1 into the conditioned medium was increased significantly by CMW 1 extract at 1h-curing, but was not changed significantly by CMW 1 extract at 7 day-curing and by the extracts of the other cements, at both curing times. The stimulating effect of CMW 1 on the secretion of TGF-β1, even with all the restrictions of an in vitro study of continuous cell lines, if confirmed in vivo, might favor the development of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis.
KW - Cement
KW - MG63
KW - Osteoblast
KW - Transforming growth factor-beta 1
UR - http://www.scopus.com/inward/record.url?scp=0036027452&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036027452&partnerID=8YFLogxK
U2 - 10.1016/S0142-9612(01)00109-0
DO - 10.1016/S0142-9612(01)00109-0
M3 - Article
C2 - 11762850
AN - SCOPUS:0036027452
SN - 0142-9612
VL - 23
SP - 305
EP - 311
JO - Biomaterials
JF - Biomaterials
IS - 1
ER -