Effect of aspirin on the fibrinolytic response in perfused rat hindquarters

Licia Iacoviello; Amalia De Curtis; Concetta Amore; Maria Cristina D'Adamo; Wlodzimierz Buczko; Giovanni de Gaetano; Maria Benedetta Donati

doi:10.1016/0014-2999(92)90283-A

Effect of aspirin on the fibrinolytic response in perfused rat hindquarters

Licia Iacoviello, Amalia De Curtis, Concetta Amore, Maria Cristina D'Adamo, Wlodzimierz Buczko, Giovanni de Gaetano, Maria Benedetta Donati

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Abstract

The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F_1α (6-keto-PGF_1α) synthesis (0.38 ± 0.09 in control and

Original language	English
Pages (from-to)	39-44
Number of pages	6
Journal	European Journal of Pharmacology
Volume	229
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(92)90283-A
Publication status	Published - Dec 8 1992

Keywords

Aspirin
Fibrinolytic activity
Venous stasis

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Pharmacology

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10.1016/0014-2999(92)90283-A

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title = "Effect of aspirin on the fibrinolytic response in perfused rat hindquarters",

abstract = "The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F1α (6-keto-PGF1α) synthesis (0.38 ± 0.09 in control and ",

keywords = "Aspirin, Fibrinolytic activity, Venous stasis",

author = "Licia Iacoviello and {De Curtis}, Amalia and Concetta Amore and D'Adamo, {Maria Cristina} and Wlodzimierz Buczko and {de Gaetano}, Giovanni and Donati, {Maria Benedetta}",

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T1 - Effect of aspirin on the fibrinolytic response in perfused rat hindquarters

AU - Iacoviello, Licia

AU - De Curtis, Amalia

AU - Amore, Concetta

AU - D'Adamo, Maria Cristina

AU - Buczko, Wlodzimierz

AU - de Gaetano, Giovanni

AU - Donati, Maria Benedetta

PY - 1992/12/8

Y1 - 1992/12/8

N2 - The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F1α (6-keto-PGF1α) synthesis (0.38 ± 0.09 in control and

AB - The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F1α (6-keto-PGF1α) synthesis (0.38 ± 0.09 in control and

KW - Aspirin

KW - Fibrinolytic activity

KW - Venous stasis

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Effect of aspirin on the fibrinolytic response in perfused rat hindquarters

Abstract

Keywords

ASJC Scopus subject areas

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