Effect of adhesive properties of buffy coat on the quality of blood components produced with Top & Top and Top & Bottom bags

Eugenio Cerelli, Martina Nocera, Erminia Di Bartolomeo, Paola Panzani, Roberto Baricchi

Research output: Contribution to journalArticlepeer-review


Background: The Transfusion Medicine Unit of Reggio Emilia currently collects whole blood using conventional quadruple Fresenius Top & Top bags. In this study, new Fresenius Top & Bottom bags were assessed and compared to the routine method with regards to product quality and operational requirements. Materials and methods: Twenty-one whole blood units were collected with both the new and the traditional bags, and then separated. Quality control data were evaluated and compared in order to estimate yield and quality of final blood components obtained with the two systems. We collected other bags, not included in the ordinary quality control programme, for comparison of platelet concentrates produced by pools of buffy coat. Results: Compared to the traditional system, the whole blood units processed with Top & Bottom bags yielded larger plasma volumes (+5.7%) and a similar amount of concentrated red blood cells, but with a much lower contamination of lymphocytes (-61.5%) and platelets (-86.6%). Consequently, the pooled platelets contained less plasma (-26.3%) and were significantly richer in platelets (+17.9%). Discussion: This study investigated the effect of centrifugation on the adhesiveness of the buffy coat to the bag used for whole blood collection. We analysed the mechanism by which this undesirable phenomenon affects the quality of packed red blood cells in two types of bags. We also documented the incomparability of measurements on platelet concentrates performed with different principles of cell counting: this vexing problem has important implications for biomedical research and for the establishment of universal product standards. Our results support the conclusion that the Top & Bottom bags produce components of higher quality than our usual system, while having equal operational efficiency. Use of the new bags could result in an important quality improvement in blood components manufacturing.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalBlood Transfusion
Issue number2
Publication statusPublished - 2015


  • Accumulated centrifugal effect
  • Adhesiveness
  • Blood buffy coat
  • Top & bottom bag
  • Top & top bag

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy


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