EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

S Paolucci; Irene Cassaniti; Federica Novazzi; L Fiorina; A Piralla; G Comolli; Raffaele Bruno; Renato Maserati; Roberto Gulminetti; Stefano Novati; Francesco Mojoli; Fausto Baldanti; Raffaele Bruno; M Mondelli; E Brunetti; A Di Matteo; E Seminari; L Maiocchi; V Zuccaro; L Pagnucco; B Mariani; S Ludovisi; R Lissandrin; A Parisi; P Sacchi; SFA Patruno; G Michelone; Roberto Gulminetti; D Zanaboni; Stefano Novati; R Maserati; P Orsolini; M Vecchia; M Sciarra; E Asperges; M Colaneri; A Di Filippo; M Sambo; S Biscarini; M Lupi; S Roda; TC Pieri; I Gallazzi; M Sachs; P Valsecchi; S Perlini; C Alfano; M Bonzano; F Briganti; G Crescenzi; AG Falchi; R Guarnone; B Guglielmana; E Maggi; I Martino; P Pettenazza; S Pioli di Marco; F Quaglia; A Sabena; F Salinaro; F Speciale; I Zunino; M De Lorenzo; G Secco; L Dimitry; G Cappa; I Maisak; B Chiodi; M Sciarrini; B Barcella; F Resta; L Moroni; G Vezzoni; L Scattaglia; E Boscolo; C Zattera; MF Tassi; V Capozza; D Vignaroli; M Bazzini; G Iotti; Francesco Mojoli; M Belliato; L Perotti; S Mongodi; G Tavazzi; G Marseglia; A Licari; I Brambilla; D Barbarini; A Bruno; P Cambieri; G Campanini; C. Cavanna; G Comolli; M Corbella; R Daturi; M Furione; P Marone; E Monzillo; S Paolucci; M Parea; E Percivalle; A Piralla; F Rovida; A Sarasini; M Zavattoni; G Adzasehoun; M Ardizzone; L Bellotti; V Brunco; E Cabano; G Casali; L Capella; D Devitis; L Dossena; G Frisco; G Garbagnoli; F Gardellini; A Girello; A Guerrizio; V Landini; C Lucchelli; V Maliardi; P Piemontese; S Pezzaia; M Premoli; C Rebuffa; C Zanello; J Bagnarino; F Bergami; A Bonetti; G Caneva; Irene Cassaniti; A Corcione; R Di Martino; A Di Napoli; A Ferrari; G Ferrari; L Fiorina; A Gallone; F Giardina; A Girardi; A Mercato; C Merla; F Novazzi; G Ratano; B Rossi; G Saveriaempillai; IM Sciabica; M Tallarita; E Vecchio Nepita; J Vitali; A Cerino; S Varchetta; B Oliviero; S Mantovani; D Mele; M Calvi; M Tizzoni; C Nicora; A Triarico; V Petronella; C Marena; A Muzzi; P Lago; S Cutti; V Novelli; F Comandatore; G BatistiBiffignandi; S Gaiarsa; M Rettani; C Bandi

doi:10.1016/j.ijid.2020.12.051

EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

S Paolucci, Irene Cassaniti, Federica Novazzi, L Fiorina, A Piralla, G Comolli, Raffaele Bruno, Renato Maserati, Roberto Gulminetti, Stefano Novati, Francesco Mojoli, Fausto Baldanti, Raffaele Bruno, M Mondelli, E Brunetti, A Di Matteo, E Seminari, L Maiocchi, V Zuccaro, L PagnuccoB Mariani, S Ludovisi, R Lissandrin, A Parisi, P Sacchi, SFA Patruno, G Michelone, Roberto Gulminetti, D Zanaboni, Stefano Novati, R Maserati, P Orsolini, M Vecchia, M Sciarra, E Asperges, M Colaneri, A Di Filippo, M Sambo, S Biscarini, M Lupi, S Roda, TC Pieri, I Gallazzi, M Sachs, P Valsecchi, S Perlini, C Alfano, M Bonzano, F Briganti, G Crescenzi, AG Falchi, R Guarnone, B Guglielmana, E Maggi, I Martino, P Pettenazza, S Pioli di Marco, F Quaglia, A Sabena, F Salinaro, F Speciale, I Zunino, M De Lorenzo, G Secco, L Dimitry, G Cappa, I Maisak, B Chiodi, M Sciarrini, B Barcella, F Resta, L Moroni, G Vezzoni, L Scattaglia, E Boscolo, C Zattera, MF Tassi, V Capozza, D Vignaroli, M Bazzini, G Iotti, Francesco Mojoli, M Belliato, L Perotti, S Mongodi, G Tavazzi, G Marseglia, A Licari, I Brambilla, D Barbarini, A Bruno, P Cambieri, G Campanini, C. Cavanna, G Comolli, M Corbella, R Daturi, M Furione, P Marone, E Monzillo, S Paolucci, M Parea, E Percivalle, A Piralla, F Rovida, A Sarasini, M Zavattoni, G Adzasehoun, M Ardizzone, L Bellotti, V Brunco, E Cabano, G Casali, L Capella, D Devitis, L Dossena, G Frisco, G Garbagnoli, F Gardellini, A Girello, A Guerrizio, V Landini, C Lucchelli, V Maliardi, P Piemontese, S Pezzaia, M Premoli, C Rebuffa, C Zanello, J Bagnarino, F Bergami, A Bonetti, G Caneva, Irene Cassaniti, A Corcione, R Di Martino, A Di Napoli, A Ferrari, G Ferrari, L Fiorina, A Gallone, F Giardina, A Girardi, A Mercato, C Merla, F Novazzi, G Ratano, B Rossi, G Saveriaempillai, IM Sciabica, M Tallarita, E Vecchio Nepita, J Vitali, A Cerino, S Varchetta, B Oliviero, S Mantovani, D Mele, M Calvi, M Tizzoni, C Nicora, A Triarico, V Petronella, C Marena, A Muzzi, P Lago, S Cutti, V Novelli, F Comandatore, G BatistiBiffignandi, S Gaiarsa, M Rettani, C Bandi

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p

Original language	English
Pages (from-to)	315-319
Number of pages	5
Journal	Int. J. Infect. Dis.
Volume	104
DOIs	https://doi.org/10.1016/j.ijid.2020.12.051
Publication status	Published - 2021

Keywords

COVID-19
EBV DNA
Lymphocyte subpopulation

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10.1016/j.ijid.2020.12.051

https://www.sciencedirect.com/science/article/pii/S1201971220325753

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Paolucci, S , Cassaniti, I, Novazzi, F, Fiorina, L, Piralla, A , Comolli, G , Bruno, R , Maserati, R , Gulminetti, R , Novati, S , Mojoli, F , Baldanti, F , Bruno, R , Mondelli, M , Brunetti, E , Di Matteo, A , Seminari, E , Maiocchi, L , Zuccaro, V , Pagnucco, L, Mariani, B, Ludovisi, S, Lissandrin, R, Parisi, A , Sacchi, P , Patruno, SFA , Michelone, G , Gulminetti, R , Zanaboni, D , Novati, S , Maserati, R , Orsolini, P , Vecchia, M, Sciarra, M, Asperges, E, Colaneri, M, Di Filippo, A, Sambo, M, Biscarini, S, Lupi, M, Roda, S, Pieri, TC, Gallazzi, I, Sachs, M, Valsecchi, P, Perlini, S, Alfano, C, Bonzano, M, Briganti, F, Crescenzi, G, Falchi, AG, Guarnone, R, Guglielmana, B, Maggi, E, Martino, I, Pettenazza, P, Pioli di Marco, S, Quaglia, F, Sabena, A, Salinaro, F, Speciale, F, Zunino, I, De Lorenzo, M, Secco, G, Dimitry, L, Cappa, G, Maisak, I, Chiodi, B, Sciarrini, M, Barcella, B, Resta, F, Moroni, L, Vezzoni, G, Scattaglia, L, Boscolo, E, Zattera, C, Tassi, MF, Capozza, V, Vignaroli, D, Bazzini, M, Iotti, G, Mojoli, F , Belliato, M, Perotti, L, Mongodi, S , Tavazzi, G , Marseglia, G , Licari, A , Brambilla, I , Barbarini, D , Bruno, A , Cambieri, P , Campanini, G , Cavanna, C , Comolli, G , Corbella, M, Daturi, R, Furione, M , Marone, P, Monzillo, E, Paolucci, S , Parea, M , Percivalle, E , Piralla, A , Rovida, F , Sarasini, A , Zavattoni, M, Adzasehoun, G, Ardizzone, M, Bellotti, L, Brunco, V, Cabano, E, Casali, G, Capella, L, Devitis, D, Dossena, L, Frisco, G, Garbagnoli, G, Gardellini, F, Girello, A, Guerrizio, A, Landini, V, Lucchelli, C, Maliardi, V, Piemontese, P, Pezzaia, S, Premoli, M, Rebuffa, C, Zanello, C, Bagnarino, J, Bergami, F, Bonetti, A, Caneva, G, Cassaniti, I , Corcione, A, Di Martino, R, Di Napoli, A, Ferrari, A, Ferrari, G, Fiorina, L, Gallone, A, Giardina, F , Girardi, A, Mercato, A, Merla, C, Novazzi, F, Ratano, G, Rossi, B, Saveriaempillai, G, Sciabica, IM, Tallarita, M, Vecchio Nepita, E, Vitali, J, Cerino, A , Varchetta, S , Oliviero, B , Mantovani, S , Mele, D, Calvi, M, Tizzoni, M, Nicora, C, Triarico, A, Petronella, V, Marena, C , Muzzi, A , Lago, P , Cutti, S , Novelli, V, Comandatore, F, BatistiBiffignandi, G, Gaiarsa, S, Rettani, M & Bandi, C 2021, 'EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count', Int. J. Infect. Dis., vol. 104, pp. 315-319. https://doi.org/10.1016/j.ijid.2020.12.051

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title = "EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count",

abstract = "Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p",

keywords = "COVID-19, EBV DNA, Lymphocyte subpopulation",

author = "S Paolucci and Irene Cassaniti and Federica Novazzi and L Fiorina and A Piralla and G Comolli and Raffaele Bruno and Renato Maserati and Roberto Gulminetti and Stefano Novati and Francesco Mojoli and Fausto Baldanti and Raffaele Bruno and M Mondelli and E Brunetti and {Di Matteo}, A and E Seminari and L Maiocchi and V Zuccaro and L Pagnucco and B Mariani and S Ludovisi and R Lissandrin and A Parisi and P Sacchi and SFA Patruno and G Michelone and Roberto Gulminetti and D Zanaboni and Stefano Novati and R Maserati and P Orsolini and M Vecchia and M Sciarra and E Asperges and M Colaneri and {Di Filippo}, A and M Sambo and S Biscarini and M Lupi and S Roda and TC Pieri and I Gallazzi and M Sachs and P Valsecchi and S Perlini and C Alfano and M Bonzano and F Briganti and G Crescenzi and AG Falchi and R Guarnone and B Guglielmana and E Maggi and I Martino and P Pettenazza and {Pioli di Marco}, S and F Quaglia and A Sabena and F Salinaro and F Speciale and I Zunino and {De Lorenzo}, M and G Secco and L Dimitry and G Cappa and I Maisak and B Chiodi and M Sciarrini and B Barcella and F Resta and L Moroni and G Vezzoni and L Scattaglia and E Boscolo and C Zattera and MF Tassi and V Capozza and D Vignaroli and M Bazzini and G Iotti and Francesco Mojoli and M Belliato and L Perotti and S Mongodi and G Tavazzi and G Marseglia and A Licari and I Brambilla and D Barbarini and A Bruno and P Cambieri and G Campanini and C. Cavanna and G Comolli and M Corbella and R Daturi and M Furione and P Marone and E Monzillo and S Paolucci and M Parea and E Percivalle and A Piralla and F Rovida and A Sarasini and M Zavattoni and G Adzasehoun and M Ardizzone and L Bellotti and V Brunco and E Cabano and G Casali and L Capella and D Devitis and L Dossena and G Frisco and G Garbagnoli and F Gardellini and A Girello and A Guerrizio and V Landini and C Lucchelli and V Maliardi and P Piemontese and S Pezzaia and M Premoli and C Rebuffa and C Zanello and J Bagnarino and F Bergami and A Bonetti and G Caneva and Irene Cassaniti and A Corcione and {Di Martino}, R and {Di Napoli}, A and A Ferrari and G Ferrari and L Fiorina and A Gallone and F Giardina and A Girardi and A Mercato and C Merla and F Novazzi and G Ratano and B Rossi and G Saveriaempillai and IM Sciabica and M Tallarita and {Vecchio Nepita}, E and J Vitali and A Cerino and S Varchetta and B Oliviero and S Mantovani and D Mele and M Calvi and M Tizzoni and C Nicora and A Triarico and V Petronella and C Marena and A Muzzi and P Lago and S Cutti and V Novelli and F Comandatore and G BatistiBiffignandi and S Gaiarsa and M Rettani and C Bandi",

year = "2021",

doi = "10.1016/j.ijid.2020.12.051",

language = "English",

volume = "104",

pages = "315--319",

journal = "Int. J. Infect. Dis.",

issn = "1201-9712",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

AU - Paolucci, S

AU - Cassaniti, Irene

AU - Novazzi, Federica

AU - Fiorina, L

AU - Piralla, A

AU - Comolli, G

AU - Bruno, Raffaele

AU - Maserati, Renato

AU - Gulminetti, Roberto

AU - Novati, Stefano

AU - Mojoli, Francesco

AU - Baldanti, Fausto

AU - Bruno, Raffaele

AU - Mondelli, M

AU - Brunetti, E

AU - Di Matteo, A

AU - Seminari, E

AU - Maiocchi, L

AU - Zuccaro, V

AU - Pagnucco, L

AU - Mariani, B

AU - Ludovisi, S

AU - Lissandrin, R

AU - Parisi, A

AU - Sacchi, P

AU - Patruno, SFA

AU - Michelone, G

AU - Gulminetti, Roberto

AU - Zanaboni, D

AU - Novati, Stefano

AU - Maserati, R

AU - Orsolini, P

AU - Vecchia, M

AU - Sciarra, M

AU - Asperges, E

AU - Colaneri, M

AU - Di Filippo, A

AU - Sambo, M

AU - Biscarini, S

AU - Lupi, M

AU - Roda, S

AU - Pieri, TC

AU - Gallazzi, I

AU - Sachs, M

AU - Valsecchi, P

AU - Perlini, S

AU - Alfano, C

AU - Bonzano, M

AU - Briganti, F

AU - Crescenzi, G

AU - Falchi, AG

AU - Guarnone, R

AU - Guglielmana, B

AU - Maggi, E

AU - Martino, I

AU - Pettenazza, P

AU - Pioli di Marco, S

AU - Quaglia, F

AU - Sabena, A

AU - Salinaro, F

AU - Speciale, F

AU - Zunino, I

AU - De Lorenzo, M

AU - Secco, G

AU - Dimitry, L

AU - Cappa, G

AU - Maisak, I

AU - Chiodi, B

AU - Sciarrini, M

AU - Barcella, B

AU - Resta, F

AU - Moroni, L

AU - Vezzoni, G

AU - Scattaglia, L

AU - Boscolo, E

AU - Zattera, C

AU - Tassi, MF

AU - Capozza, V

AU - Vignaroli, D

AU - Bazzini, M

AU - Iotti, G

AU - Mojoli, Francesco

AU - Belliato, M

AU - Perotti, L

AU - Mongodi, S

AU - Tavazzi, G

AU - Marseglia, G

AU - Licari, A

AU - Brambilla, I

AU - Barbarini, D

AU - Bruno, A

AU - Cambieri, P

AU - Campanini, G

AU - Cavanna, C.

AU - Comolli, G

AU - Corbella, M

AU - Daturi, R

AU - Furione, M

AU - Marone, P

AU - Monzillo, E

AU - Paolucci, S

AU - Parea, M

AU - Percivalle, E

AU - Piralla, A

AU - Rovida, F

AU - Sarasini, A

AU - Zavattoni, M

AU - Adzasehoun, G

AU - Ardizzone, M

AU - Bellotti, L

AU - Brunco, V

AU - Cabano, E

AU - Casali, G

AU - Capella, L

AU - Devitis, D

AU - Dossena, L

AU - Frisco, G

AU - Garbagnoli, G

AU - Gardellini, F

AU - Girello, A

AU - Guerrizio, A

AU - Landini, V

AU - Lucchelli, C

AU - Maliardi, V

AU - Piemontese, P

AU - Pezzaia, S

AU - Premoli, M

AU - Rebuffa, C

AU - Zanello, C

AU - Bagnarino, J

AU - Bergami, F

AU - Bonetti, A

AU - Caneva, G

AU - Cassaniti, Irene

AU - Corcione, A

AU - Di Martino, R

AU - Di Napoli, A

AU - Ferrari, A

AU - Ferrari, G

AU - Fiorina, L

AU - Gallone, A

AU - Giardina, F

AU - Girardi, A

AU - Mercato, A

AU - Merla, C

AU - Novazzi, F

AU - Ratano, G

AU - Rossi, B

AU - Saveriaempillai, G

AU - Sciabica, IM

AU - Tallarita, M

AU - Vecchio Nepita, E

AU - Vitali, J

AU - Cerino, A

AU - Varchetta, S

AU - Oliviero, B

AU - Mantovani, S

AU - Mele, D

AU - Calvi, M

AU - Tizzoni, M

AU - Nicora, C

AU - Triarico, A

AU - Petronella, V

AU - Marena, C

AU - Muzzi, A

AU - Lago, P

AU - Cutti, S

AU - Novelli, V

AU - Comandatore, F

AU - BatistiBiffignandi, G

AU - Gaiarsa, S

AU - Rettani, M

AU - Bandi, C

PY - 2021

Y1 - 2021

N2 - Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p

AB - Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p

KW - COVID-19

KW - EBV DNA

KW - Lymphocyte subpopulation

U2 - 10.1016/j.ijid.2020.12.051

DO - 10.1016/j.ijid.2020.12.051

M3 - Article

SN - 1201-9712

VL - 104

SP - 315

EP - 319

JO - Int. J. Infect. Dis.

JF - Int. J. Infect. Dis.

ER -

EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

Abstract

Keywords

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