Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line

Maria Gabriella Caruso; Caterina Messa; Antonella Orlando; Benedetta D'Attoma; Maria Notarnicola

doi:10.1016/j.fitote.2008.05.003

Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line

Maria Gabriella Caruso, Caterina Messa, Antonella Orlando, Benedetta D'Attoma, Maria Notarnicola

Ente Ospedaliero Specializzato in Gastroenterologia "Saverio de Bellis"

Research output: Contribution to journal › Article › peer-review

Abstract

The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 μM to 50 μM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer.

Original language	English
Pages (from-to)	524-528
Number of pages	5
Journal	Fitoterapia
Volume	79
Issue number	7-8
DOIs	https://doi.org/10.1016/j.fitote.2008.05.003
Publication status	Published - Dec 2008

Keywords

Colon cancer
Genistein
HMGCoA reductase
LDL receptor

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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10.1016/j.fitote.2008.05.003

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title = "Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line",

abstract = "The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 μM to 50 μM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer.",

keywords = "Colon cancer, Genistein, HMGCoA reductase, LDL receptor",

author = "Caruso, {Maria Gabriella} and Caterina Messa and Antonella Orlando and Benedetta D'Attoma and Maria Notarnicola",

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T1 - Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line

AU - Caruso, Maria Gabriella

AU - Messa, Caterina

AU - Orlando, Antonella

AU - D'Attoma, Benedetta

AU - Notarnicola, Maria

PY - 2008/12

Y1 - 2008/12

N2 - The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 μM to 50 μM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer.

AB - The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 μM to 50 μM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer.

KW - Colon cancer

KW - Genistein

KW - HMGCoA reductase

KW - LDL receptor

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Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line

Abstract

Keywords

ASJC Scopus subject areas

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