TY - JOUR
T1 - Down-regulation of DPH2L gene during cellular differentiation /apoptosis
T2 - Use of mRNA differential display
AU - Liu, Guizhong
AU - Ferrari, Nicoletta
AU - Levi, Giovanni
AU - Wu, Min
PY - 1999/3
Y1 - 1999/3
N2 - To characterize the genes associated with differentiation/apoptosis induced by all-trons retinoic acid (ATRA) in human lung cancer cells, mRNA differential display was employed. Six cDNA fragments have been isolated, and one of them corresponds to a sequence-known gene DPH2L with unknown function, which was first isolated from the critical region of deletion on chromosome 17p13.3 in human ovarian carcinoma, and regarded as a candidate tumor suppressor gene. Results show that DPH2L is a wide expressed gene, and has another major transcript besides the previously reported 2.3 kb transcript. It is proved that the DPH2L gene is down-regulated during differentiation or apoptosis in several kinds of cancer cells induced by all-trans retinoic acid and N-(4-hydroxyphenyl) retinamide (4HPR). This may suggest that DPH2L does not play a role as a tumor suppressor gene, on the contrary, its down-regulation may be functionally involved in the transition from cell growth to differentiation or apoptosis.
AB - To characterize the genes associated with differentiation/apoptosis induced by all-trons retinoic acid (ATRA) in human lung cancer cells, mRNA differential display was employed. Six cDNA fragments have been isolated, and one of them corresponds to a sequence-known gene DPH2L with unknown function, which was first isolated from the critical region of deletion on chromosome 17p13.3 in human ovarian carcinoma, and regarded as a candidate tumor suppressor gene. Results show that DPH2L is a wide expressed gene, and has another major transcript besides the previously reported 2.3 kb transcript. It is proved that the DPH2L gene is down-regulated during differentiation or apoptosis in several kinds of cancer cells induced by all-trans retinoic acid and N-(4-hydroxyphenyl) retinamide (4HPR). This may suggest that DPH2L does not play a role as a tumor suppressor gene, on the contrary, its down-regulation may be functionally involved in the transition from cell growth to differentiation or apoptosis.
KW - Apoptosis
KW - Cell differentiation
KW - mRNA differential display
KW - Retinoic acids
KW - Tumor suppressor gene
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M3 - Article
AN - SCOPUS:5844407899
SN - 1001-6538
VL - 44
SP - 496
EP - 503
JO - Chinese Science Bulletin
JF - Chinese Science Bulletin
IS - 6
ER -