TY - JOUR
T1 - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
AU - Bonassi, Stefano
AU - Ceppi, Marcello
AU - Møller, Peter
AU - Azqueta, Amaya
AU - Milić, Mirta
AU - Neri, Monica
AU - Brunborg, Gunnar
AU - Godschalk, Roger
AU - Koppen, Gudrun
AU - Langie, Sabine A S
AU - Teixeira, João Paulo
AU - Bruzzone, Marco
AU - Da Silva, Juliana
AU - Benedetti, Danieli
AU - Cavallo, Delia
AU - Ursini, Cinzia Lucia
AU - Giovannelli, Lisa
AU - Moretti, Silvia
AU - Riso, Patrizia
AU - Del Bo', Cristian
AU - Russo, Patrizia
AU - Dobrzyńska, Malgorzata
AU - Goroshinskaya, Irina A
AU - Surikova, Ekaterina I
AU - Staruchova, Marta
AU - Barančokova, Magdalena
AU - Volkovova, Katarina
AU - Kažimirova, Alena
AU - Smolkova, Bozena
AU - Laffon, Blanca
AU - Valdiglesias, Vanessa
AU - Pastor, Susana
AU - Marcos, Ricard
AU - Hernández, Alba
AU - Gajski, Goran
AU - Spremo-Potparević, Biljana
AU - Živković, Lada
AU - Boutet-Robinet, Elisa
AU - Perdry, Hervé
AU - Lebailly, Pierre
AU - Perez, Carlos L
AU - Basaran, Nursen
AU - Nemeth, Zsuzsanna
AU - Safar, Anna
AU - Dusinska, Maria
AU - Collins, Andrew
AU - hCOMET project
N1 - © 2021. The Author(s).
PY - 2021/8/18
Y1 - 2021/8/18
N2 - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
AB - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
KW - Cell-Free Nucleic Acids/genetics
KW - Comet Assay
KW - DNA Damage/genetics
KW - Humans
KW - Kaplan-Meier Estimate
KW - Leukocytes/pathology
KW - Neoplasms/genetics
KW - Proportional Hazards Models
U2 - 10.1038/s41598-021-95976-7
DO - 10.1038/s41598-021-95976-7
M3 - Article
C2 - 34408182
SN - 2045-2322
VL - 11
SP - 16793
JO - Sci. Rep.
JF - Sci. Rep.
IS - 1
ER -