Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis; Riccardo Paggi; Thomas J. Lowery; Jessica L. Snyder; Giulia Menchinelli; Maurizio Sanguinetti; Brunella Posteraro; Antonella Mencacci

doi:10.3390/antibiotics10080950

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis, Riccardo Paggi, Thomas J. Lowery, Jessica L. Snyder, Giulia Menchinelli, Maurizio Sanguinetti, Brunella Posteraro, Antonella Mencacci

IRCCS Fondazione Policlinico Universitario A. Gemelli

Research output: Contribution to journal › Article › peer-review

Abstract

Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

Original language	English
Article number	950
Journal	Antibiotics
Volume	10
Issue number	8
DOIs	https://doi.org/10.3390/antibiotics10080950
Publication status	Published - Aug 2021

Keywords

Antimicrobial resistance
Blood sample
Bloodstream infection
Direct detection
KPC carbapenemase
Magnetic resonance
T2Resistance panel

ASJC Scopus subject areas

Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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10.3390/antibiotics10080950

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@article{94d7fce0aad14b8ba3400399fc6b3c7e,

title = "Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay",

abstract = "Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance{\texttrademark} panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance{\texttrademark} panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.",

keywords = "Antimicrobial resistance, Blood sample, Bloodstream infection, Direct detection, KPC carbapenemase, Magnetic resonance, T2Resistance panel",

author = "Angelis, {Giulia De} and Riccardo Paggi and Lowery, {Thomas J.} and Snyder, {Jessica L.} and Giulia Menchinelli and Maurizio Sanguinetti and Brunella Posteraro and Antonella Mencacci",

note = "Funding Information: Funding: The study was supported by the Universit{\`a} Cattolica del Sacro Cuore, Rome, Italy (research grant Linea D1). Funding Information: The study was supported by the Universit? Cattolica del Sacro Cuore, Rome, Italy (research grant Linea D1).We thank T2Biosystems, Inc., Lexington, MA, USA for providing the T2Resistance? panel cartridges and test reagents. T2Biosystems, Inc. had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. We are indebted to Franziska Michaela Lohmeyer for providing English language assistance. The study was presented in part at the 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) in Amsterdam, Netherlands, 13?16 April 2019. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = aug,

doi = "10.3390/antibiotics10080950",

language = "English",

volume = "10",

journal = "Antibiotics",

issn = "2079-6382",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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T1 - Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

AU - Angelis, Giulia De

AU - Paggi, Riccardo

AU - Lowery, Thomas J.

AU - Snyder, Jessica L.

AU - Menchinelli, Giulia

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

AU - Mencacci, Antonella

N1 - Funding Information: Funding: The study was supported by the Università Cattolica del Sacro Cuore, Rome, Italy (research grant Linea D1). Funding Information: The study was supported by the Universit? Cattolica del Sacro Cuore, Rome, Italy (research grant Linea D1).We thank T2Biosystems, Inc., Lexington, MA, USA for providing the T2Resistance? panel cartridges and test reagents. T2Biosystems, Inc. had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. We are indebted to Franziska Michaela Lohmeyer for providing English language assistance. The study was presented in part at the 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) in Amsterdam, Netherlands, 13?16 April 2019. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/8

Y1 - 2021/8

N2 - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

AB - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

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DO - 10.3390/antibiotics10080950

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JF - Antibiotics

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ER -

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Abstract

Keywords

ASJC Scopus subject areas

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