TY - JOUR
T1 - Differential responsiveness to constitutive vs. inducible chemokines of immature and mature mouse dendritic cells
AU - Vecchi, Annunciata
AU - Massimiliano, Lucia
AU - Ramponi, Simona
AU - Luini, Walter
AU - Bernasconi, Sergio
AU - Bonecchi, Raffaella
AU - Allavena, Paola
AU - Parmentier, Marc
AU - Mantovani, Alberto
AU - Sozzani, Silvano
PY - 1999
Y1 - 1999
N2 - Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultured with granulocyte-macrophage-CSF and Flt3 ligand for 9 days. Chemokines active on immature DC include MIP1α, RANTES, MIP1β, MCP-1, MCP-3, and the constitutively expressed SDF1, MDC, and ELC. TNF-α-induced DC maturation caused reduction of migration to inducible chemokines (MIP1α, RANTES, MIP1β, MCP-1, and MCP-3) and increased migration to SDF1, MDC, and ELC. Similar results were obtained by CD40 ligation or culture in the presence of bacterial lipopolysaccharide. TNF-α down- regulated CC chemokine receptor (CCR)1, CCR2, and CCR5 and up-regulated CCR7 mRNA levels, in agreement with functional data. This study shows that selective responsiveness of mature and immature DC to inducible vs. constitutively produced chemokines can contribute to the regulated trafficking of DC.
AB - Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultured with granulocyte-macrophage-CSF and Flt3 ligand for 9 days. Chemokines active on immature DC include MIP1α, RANTES, MIP1β, MCP-1, MCP-3, and the constitutively expressed SDF1, MDC, and ELC. TNF-α-induced DC maturation caused reduction of migration to inducible chemokines (MIP1α, RANTES, MIP1β, MCP-1, and MCP-3) and increased migration to SDF1, MDC, and ELC. Similar results were obtained by CD40 ligation or culture in the presence of bacterial lipopolysaccharide. TNF-α down- regulated CC chemokine receptor (CCR)1, CCR2, and CCR5 and up-regulated CCR7 mRNA levels, in agreement with functional data. This study shows that selective responsiveness of mature and immature DC to inducible vs. constitutively produced chemokines can contribute to the regulated trafficking of DC.
KW - CD34-derived DC
KW - Chemokime receptors
KW - Chemotaxis
KW - Transmigration
UR - http://www.scopus.com/inward/record.url?scp=0032841695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032841695&partnerID=8YFLogxK
M3 - Article
C2 - 10496320
AN - SCOPUS:0032841695
SN - 0741-5400
VL - 66
SP - 489
EP - 494
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 3
ER -