Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment

Laura Pedranzini; Federica Mottadelli; Simona Ronzoni; Franca Rossella; Manuela Ferracin; Ivana Magnani; Gaia Roversi; Patrizia Colapietro; Massimo Negrini; Pier Giuseppe Pelicci; Lidia Larizza

doi:10.1016/j.leukres.2010.02.012

Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment

Laura Pedranzini, Federica Mottadelli, Simona Ronzoni, Franca Rossella, Manuela Ferracin, Ivana Magnani, Gaia Roversi, Patrizia Colapietro, Massimo Negrini, Pier Giuseppe Pelicci, Lidia Larizza

Research output: Contribution to journal › Article › peer-review

Abstract

The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34⁺CD38^- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34⁺CD38^- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34⁺CD38^- fraction.

Original language	English
Pages (from-to)	1287-1295
Number of pages	9
Journal	Leukemia Research
Volume	34
Issue number	10
DOIs	https://doi.org/10.1016/j.leukres.2010.02.012
Publication status	Published - Oct 2010

Keywords

AML
CD34CD38
Cytogenomic characterization
Kasumi-1
MiRNA expression

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

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10.1016/j.leukres.2010.02.012

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title = "Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment",

abstract = "The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.",

keywords = "AML, CD34CD38, Cytogenomic characterization, Kasumi-1, MiRNA expression",

author = "Laura Pedranzini and Federica Mottadelli and Simona Ronzoni and Franca Rossella and Manuela Ferracin and Ivana Magnani and Gaia Roversi and Patrizia Colapietro and Massimo Negrini and Pelicci, {Pier Giuseppe} and Lidia Larizza",

year = "2010",

month = oct,

doi = "10.1016/j.leukres.2010.02.012",

language = "English",

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T1 - Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment

AU - Pedranzini, Laura

AU - Mottadelli, Federica

AU - Ronzoni, Simona

AU - Rossella, Franca

AU - Ferracin, Manuela

AU - Magnani, Ivana

AU - Roversi, Gaia

AU - Colapietro, Patrizia

AU - Negrini, Massimo

AU - Pelicci, Pier Giuseppe

AU - Larizza, Lidia

PY - 2010/10

Y1 - 2010/10

N2 - The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.

AB - The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.

KW - AML

KW - CD34CD38

KW - Cytogenomic characterization

KW - Kasumi-1

KW - MiRNA expression

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DO - 10.1016/j.leukres.2010.02.012

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SN - 0145-2126

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EP - 1295

JO - Leukemia Research

JF - Leukemia Research

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Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment

Abstract

Keywords

ASJC Scopus subject areas

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