Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases

Bernhard Gentner; Axel Wein; Roland S. Croner; Isabel Zeittraeger; Ralph M. Wirtz; Franz Roedel; Arno Dimmler; Laure Dorlaque; Werner Hohenberger; Eckhart G. Hahn; Wolfgang M. Brueckl

Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases

Bernhard Gentner, Axel Wein, Roland S. Croner, Isabel Zeittraeger, Ralph M. Wirtz, Franz Roedel, Arno Dimmler, Laure Dorlaque, Werner Hohenberger, Eckhart G. Hahn, Wolfgang M. Brueckl

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been strongly implicated in the pathogenesis of many types of human cancer. We wanted to specifically define their role in established colorectal cancer liver metastases. Patients and Methods: The MMP/TIMP expression profiles of N=9 colorectal primary tumour liver metastasis tissue pairs were determined using oligonucleotide-based arrays. Expression levels for the most relevant MMPs were confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Additionally, unsupervised clustering using the MMP/TIMP profile of N=25 colorectal cancer liver metastases was performed and the response to palliative 5-fluorouracil (5-FU)-based chemotherapy was assessed using radiological response criteria. Results: When comparing the primary tumors to their synchronous liver metastases, a statistically significant (p

Original language	English
Pages (from-to)	67-74
Number of pages	8
Journal	Anticancer Research
Volume	29
Issue number	1
Publication status	Published - Jan 2009

Keywords

Colorectal carcinoma (crc)
Inhibitor of matrix metalloproteinase (timp)
Matrix metalloproteinase (mmp)
Microarray analysis

ASJC Scopus subject areas

Cancer Research
Oncology

Cite this

Gentner, B., Wein, A., Croner, R. S., Zeittraeger, I., Wirtz, R. M., Roedel, F., Dimmler, A., Dorlaque, L., Hohenberger, W., Hahn, E. G., & Brueckl, W. M. (2009). Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases. Anticancer Research, 29(1), 67-74.

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title = "Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases",

abstract = "Background: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been strongly implicated in the pathogenesis of many types of human cancer. We wanted to specifically define their role in established colorectal cancer liver metastases. Patients and Methods: The MMP/TIMP expression profiles of N=9 colorectal primary tumour liver metastasis tissue pairs were determined using oligonucleotide-based arrays. Expression levels for the most relevant MMPs were confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Additionally, unsupervised clustering using the MMP/TIMP profile of N=25 colorectal cancer liver metastases was performed and the response to palliative 5-fluorouracil (5-FU)-based chemotherapy was assessed using radiological response criteria. Results: When comparing the primary tumors to their synchronous liver metastases, a statistically significant (p",

keywords = "Colorectal carcinoma (crc), Inhibitor of matrix metalloproteinase (timp), Matrix metalloproteinase (mmp), Microarray analysis",

author = "Bernhard Gentner and Axel Wein and Croner, {Roland S.} and Isabel Zeittraeger and Wirtz, {Ralph M.} and Franz Roedel and Arno Dimmler and Laure Dorlaque and Werner Hohenberger and Hahn, {Eckhart G.} and Brueckl, {Wolfgang M.}",

year = "2009",

month = jan,

language = "English",

volume = "29",

pages = "67--74",

journal = "Anticancer Research",

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publisher = "International Institute of Anticancer Research",

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T1 - Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases

AU - Gentner, Bernhard

AU - Wein, Axel

AU - Croner, Roland S.

AU - Zeittraeger, Isabel

AU - Wirtz, Ralph M.

AU - Roedel, Franz

AU - Dimmler, Arno

AU - Dorlaque, Laure

AU - Hohenberger, Werner

AU - Hahn, Eckhart G.

AU - Brueckl, Wolfgang M.

PY - 2009/1

Y1 - 2009/1

N2 - Background: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been strongly implicated in the pathogenesis of many types of human cancer. We wanted to specifically define their role in established colorectal cancer liver metastases. Patients and Methods: The MMP/TIMP expression profiles of N=9 colorectal primary tumour liver metastasis tissue pairs were determined using oligonucleotide-based arrays. Expression levels for the most relevant MMPs were confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Additionally, unsupervised clustering using the MMP/TIMP profile of N=25 colorectal cancer liver metastases was performed and the response to palliative 5-fluorouracil (5-FU)-based chemotherapy was assessed using radiological response criteria. Results: When comparing the primary tumors to their synchronous liver metastases, a statistically significant (p

AB - Background: Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been strongly implicated in the pathogenesis of many types of human cancer. We wanted to specifically define their role in established colorectal cancer liver metastases. Patients and Methods: The MMP/TIMP expression profiles of N=9 colorectal primary tumour liver metastasis tissue pairs were determined using oligonucleotide-based arrays. Expression levels for the most relevant MMPs were confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Additionally, unsupervised clustering using the MMP/TIMP profile of N=25 colorectal cancer liver metastases was performed and the response to palliative 5-fluorouracil (5-FU)-based chemotherapy was assessed using radiological response criteria. Results: When comparing the primary tumors to their synchronous liver metastases, a statistically significant (p

KW - Colorectal carcinoma (crc)

KW - Inhibitor of matrix metalloproteinase (timp)

KW - Matrix metalloproteinase (mmp)

KW - Microarray analysis

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SN - 0250-7005

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JF - Anticancer Research

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Differences In the Gene Expression Profile of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases

Abstract

Keywords

ASJC Scopus subject areas

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