Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in "Selected" Diagnostic Practice

Paola Parente; Claudia Covelli; Magda Zanelli; Domenico Trombetta; Illuminato Carosi; Cristiano Carbonelli; Marco Sperandeo; Luca Mastracci; Giovanni Biancofiore; Maurizio Zizzo; Marco Taurchini; Stefano Ascani; Paolo Graziano

doi:10.3390/diagnostics10100748

Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in "Selected" Diagnostic Practice

Paola Parente, Claudia Covelli, Magda Zanelli, Domenico Trombetta, Illuminato Carosi, Cristiano Carbonelli, Marco Sperandeo, Luca Mastracci, Giovanni Biancofiore, Maurizio Zizzo, Marco Taurchini, Stefano Ascani, Paolo Graziano

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis.

METHODS: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy.

RESULTS: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique.

CONCLUSIONS: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.

Original language	English
Article number	748
Journal	Diagn.
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/diagnostics10100748
Publication status	Published - Sept 25 2020

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@article{3fde7eb552d44925963569176064dc39,

title = "Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in {"}Selected{"} Diagnostic Practice",

abstract = "BACKGROUND: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis.METHODS: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy.RESULTS: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique.CONCLUSIONS: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.",

author = "Paola Parente and Claudia Covelli and Magda Zanelli and Domenico Trombetta and Illuminato Carosi and Cristiano Carbonelli and Marco Sperandeo and Luca Mastracci and Giovanni Biancofiore and Maurizio Zizzo and Marco Taurchini and Stefano Ascani and Paolo Graziano",

year = "2020",

month = sep,

day = "25",

doi = "10.3390/diagnostics10100748",

language = "English",

volume = "10",

journal = "Diagn.",

issn = "2075-4418",

publisher = "MDPI AG",

number = "10",

}

TY - JOUR

T1 - Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in "Selected" Diagnostic Practice

AU - Parente, Paola

AU - Covelli, Claudia

AU - Zanelli, Magda

AU - Trombetta, Domenico

AU - Carosi, Illuminato

AU - Carbonelli, Cristiano

AU - Sperandeo, Marco

AU - Mastracci, Luca

AU - Biancofiore, Giovanni

AU - Zizzo, Maurizio

AU - Taurchini, Marco

AU - Ascani, Stefano

AU - Graziano, Paolo

PY - 2020/9/25

Y1 - 2020/9/25

N2 - BACKGROUND: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis.METHODS: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy.RESULTS: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique.CONCLUSIONS: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.

AB - BACKGROUND: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis.METHODS: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy.RESULTS: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique.CONCLUSIONS: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.

U2 - 10.3390/diagnostics10100748

DO - 10.3390/diagnostics10100748

M3 - Article

C2 - 32992679

SN - 2075-4418

VL - 10

JO - Diagn.

JF - Diagn.

IS - 10

M1 - 748

ER -

Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in "Selected" Diagnostic Practice

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