TY - JOUR
T1 - Determination of urinary ortho- and meta-cresol in humans by headspace SPME gas chromatography/mass spectrometry
AU - Fustinoni, Silvia
AU - Mercadante, Rosa
AU - Campo, Laura
AU - Scibetta, Licia
AU - Valla, Carla
AU - Foà, Vito
PY - 2005/3/25
Y1 - 2005/3/25
N2 - ortho-Cresol (o-C) and meta-cresol (m-C) are minor urinary metabolites of toluene, a widely used chemical with neurotoxicological properties. A new assay for their determination in human urine is here proposed. Urinary cresol sulphates and glucuronates are submitted to acid hydrolysis, urine is neutralized, added with o-cresols-d8, and analytes are sampled in the headspace of urine by SPME using a polydimethylsiloxane fiber. Analysis is performed by GC/MS using, for separation, either a SupelcoWax10 (for o-C) or a chiral CP Cresol (for o-C and m-C) column. The method is very specific, with a range of linearity 0-5.0 mg/l, within- and between-run precision, as coefficient of variation,
AB - ortho-Cresol (o-C) and meta-cresol (m-C) are minor urinary metabolites of toluene, a widely used chemical with neurotoxicological properties. A new assay for their determination in human urine is here proposed. Urinary cresol sulphates and glucuronates are submitted to acid hydrolysis, urine is neutralized, added with o-cresols-d8, and analytes are sampled in the headspace of urine by SPME using a polydimethylsiloxane fiber. Analysis is performed by GC/MS using, for separation, either a SupelcoWax10 (for o-C) or a chiral CP Cresol (for o-C and m-C) column. The method is very specific, with a range of linearity 0-5.0 mg/l, within- and between-run precision, as coefficient of variation,
KW - Biological monitoring
KW - Occupational exposure to toluene
KW - Urinary cresols
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U2 - 10.1016/j.jchromb.2004.12.029
DO - 10.1016/j.jchromb.2004.12.029
M3 - Article
C2 - 15687000
AN - SCOPUS:15944400347
SN - 1570-0232
VL - 817
SP - 309
EP - 317
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -