Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR

E. Tamburrini; E. Ortona; E. Visconti; P. Margutti; P. Mencarini; M. Zolfo; S. Marinaci; A. Siracusano

Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR

E. Tamburrini, E. Ortona, E. Visconti, P. Margutti, P. Mencarini, M. Zolfo, S. Marinaci, A. Siracusano

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested. PCR.

Original language	English
Journal	Journal of Eukaryotic Microbiology
Volume	44
Issue number	6
Publication status	Published - Nov 1997

Keywords

Nested PCR
Oropharyngeal washings
PCR-SHELA
Pneumocystis carinii DNA
Polymerase chain reaction

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Agricultural and Biological Sciences (miscellaneous)
Applied Microbiology and Biotechnology
Microbiology

Cite this

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title = "Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR",

abstract = "Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested. PCR.",

keywords = "Nested PCR, Oropharyngeal washings, PCR-SHELA, Pneumocystis carinii DNA, Polymerase chain reaction",

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T1 - Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR

AU - Tamburrini, E.

AU - Ortona, E.

AU - Visconti, E.

AU - Margutti, P.

AU - Mencarini, P.

AU - Zolfo, M.

AU - Marinaci, S.

AU - Siracusano, A.

PY - 1997/11

Y1 - 1997/11

N2 - Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested. PCR.

AB - Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested. PCR.

KW - Nested PCR

KW - Oropharyngeal washings

KW - PCR-SHELA

KW - Pneumocystis carinii DNA

KW - Polymerase chain reaction

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Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR

Abstract

Keywords

ASJC Scopus subject areas

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