Detection of micrometastatic cells in breast cancer by RT-PCR for the mammaglobin gene

Patrizia Bossolasco, Clara Ricci, Gabriella Farina, Davide Soligo, Donata Pedretti, Alberto Scanni, Giorgio Lambertenghi Deliliers

Research output: Contribution to journalArticlepeer-review

Abstract

The detection of circulating cancer cells in the bone marrow (BM) and peripheral blood (PB) of patients with solid tumors may be useful for disease staging. To this aim, we evaluated the expression of the mammaglobin gene by reverse transcriptase polymerase chain reaction (RT-PCR) in 60 patients with breast cancer. Moreover, several controls were examined to test the specificity of this marker. The positive cases included 23.6% of the patients with and 9% of those without metastasis. Only 4/60 negative controls analyzed were positive by PCR. Our results show high specificity and a good correlation with disease status.

Original languageEnglish
Pages (from-to)60-63
Number of pages4
JournalCancer Detection and Prevention
Volume26
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • Breast neoplasm
  • Mammaglobin
  • Neoplasm circulating cells
  • RT-PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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