Detection of high molecular weight proteins by MALDI imaging mass spectrometry

Veronica Mainini, Giorgio Bovo, Clizia Chinello, Erica Gianazza, Marco Grasso, Giorgio Cattoretti, Fulvio Magni

Research output: Contribution to journalArticlepeer-review


MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.

Original languageEnglish
Pages (from-to)1101-1107
Number of pages7
JournalMolecular BioSystems
Issue number6
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Medicine(all)


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