Detection of bovine leukaemia virus (BLV) infection by DNA probe technology

Simona Gaudi, Wilma Ponti, Alessandra Agresti, Raffaella Meneveri, Massimo Malcovati, Luigi Bonizzi, Giorgio Poli, Aldo Amato, Enrico Ginelli

Research output: Contribution to journalArticlepeer-review


Classical serological methods and Southern blot hybridization for the diagnosis of bovine leukaemia virus (BLV) infection have been compared during the first nine months of life of offspring from BLV serum-negative and serum-positive dams belonging to a Friesian dairy herd in Italy. At birth, 9/13 calves analysed showed serum positivity for anti-gp60 BLV antibodies by agar immunodiffusion and/or by ELISA. However, only two calves were positive for BLV integrated proviruses in their lymphocyte DNA. At six months of age, anti-gp60 BLV antibodies and proviral DNA positivities were simultaneously shown only by the two cattle identified as DNA-positive at birth. This pattern remained constant up to nine months of age. Furthermore, analysis of the molecular characteristics of BLV integrated proviruses, carried out by using, as probes, the almost complete proviral genome (Belgian isolate) or a subclone of the env gene radioactively labelled or chemically modified, revealed that the calves under study were infected by a different isolate (Japanese isolate) and that, in one of the cattle, the majority of integrated proviruses was characterized by deletions probably located in the 5′ half of the proviral genome.

Original languageEnglish
Pages (from-to)163-174
Number of pages12
JournalMolecular and Cellular Probes
Issue number3
Publication statusPublished - 1990


  • bovine leukaemia virus
  • DNA hybridization
  • DNA probe
  • early diagnosis
  • Enzootic bovine leukosis
  • viral infection

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology


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