TY - GEN
T1 - Design of a microfluidic device for the phase-contrast tomography of flowing cells
AU - Villone, Massimiliano M.
AU - Santonastaso, Erica
AU - Memmolo, Pasquale
AU - Trotta, Gianluca
AU - Merola, Francesco
AU - Maffettone, Pier Luca
AU - Ferraro, Pietro
N1 - Funding Information:
The research has been partly funded by project PRIN 2017, Morphological Biomarkers for early diagnosis in Oncology (MORFEO) Prto. 2017N7R2CJ.
Publisher Copyright:
© 2021 SPIE
PY - 2021
Y1 - 2021
N2 - The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.
AB - The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.
KW - Liquid biopsy
KW - Microfluidics
KW - Tomographic phase microscopy
UR - http://www.scopus.com/inward/record.url?scp=85109751326&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85109751326&partnerID=8YFLogxK
U2 - 10.1117/12.2592327
DO - 10.1117/12.2592327
M3 - Conference contribution
AN - SCOPUS:85109751326
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V
A2 - Ferraro, Pietro
A2 - Grilli, Simonetta
A2 - Ritsch-Marte, Monika
A2 - Hitzenberger, Christoph K.
PB - SPIE
T2 - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
Y2 - 21 June 2021 through 25 June 2021
ER -