Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

R. Dorati; A. De Trizio; S. Marconi; A. Ferrara; F. Auricchio; I. Genta; T. Modena; M. Benazzo; A. Benazzo; G. Volpato; B. Conti

doi:10.1002/mabi.201600426 [doi]

Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

R. Dorati, A. De Trizio, S. Marconi, A. Ferrara, F. Auricchio, I. Genta, T. Modena, M. Benazzo, A. Benazzo, G. Volpato, B. Conti

Research output: Contribution to journal › Article › peer-review

Abstract

A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M w . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).

Original language	English
Pages (from-to)	10.1002/mabi.201600426. Epub 2017 Jan 27
Journal	Macromolecular Bioscience
Volume	17
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201600426 [doi]
Publication status	Published - Jun 1 2017

Keywords

biopolymers
chitosan
polylactide-co-polycaprolactone
scaffold
tissue regeneration

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10.1002/mabi.201600426 [doi]

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title = "Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering",

abstract = "A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M w . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).",

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author = "R. Dorati and Trizio, {A. De} and S. Marconi and A. Ferrara and F. Auricchio and I. Genta and T. Modena and M. Benazzo and A. Benazzo and G. Volpato and B. Conti",

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T1 - Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

AU - Dorati, R.

AU - Trizio, A. De

AU - Marconi, S.

AU - Ferrara, A.

AU - Auricchio, F.

AU - Genta, I.

AU - Modena, T.

AU - Benazzo, M.

AU - Benazzo, A.

AU - Volpato, G.

AU - Conti, B.

N1 - LR: 20170614; CI: (c) 2017; JID: 101135941; OTO: NOTNLM; 2016/10/11 00:00 [received]; 2016/12/22 00:00 [revised]; 2017/01/28 06:00 [pubmed]; 2017/01/28 06:00 [medline]; 2017/01/28 06:00 [entrez]; ppublish

PY - 2017/6/1

Y1 - 2017/6/1

N2 - A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M w . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).

AB - A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M w . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).

KW - biopolymers

KW - chitosan

KW - polylactide-co-polycaprolactone

KW - scaffold

KW - tissue regeneration

U2 - 10.1002/mabi.201600426 [doi]

DO - 10.1002/mabi.201600426 [doi]

M3 - Article

SN - 1616-5187

VL - 17

SP - 10.1002/mabi.201600426. Epub 2017 Jan 27

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 6

ER -

Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

Abstract

Keywords

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