DADLE induces a reversible hibernation-like state in HeLa cells

Lorella Vecchio, Cristiana Soldani, Maria Grazia Bottone, Manuela Malatesta, Terence E. Martin, Lawrence I. Rothblum, Carlo Pellicciari, Marco Biggiogera

Research output: Contribution to journalArticlepeer-review


[D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalHistochemistry and Cell Biology
Issue number1-2
Publication statusPublished - Jan 2006


  • Electron microscopy
  • Induced hypometabolic state
  • Nucleus
  • Proliferation
  • Transcription

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation


Dive into the research topics of 'DADLE induces a reversible hibernation-like state in HeLa cells'. Together they form a unique fingerprint.

Cite this