TY - JOUR
T1 - DADLE induces a reversible hibernation-like state in HeLa cells
AU - Vecchio, Lorella
AU - Soldani, Cristiana
AU - Bottone, Maria Grazia
AU - Malatesta, Manuela
AU - Martin, Terence E.
AU - Rothblum, Lawrence I.
AU - Pellicciari, Carlo
AU - Biggiogera, Marco
PY - 2006/1
Y1 - 2006/1
N2 - [D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.
AB - [D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.
KW - Electron microscopy
KW - Induced hypometabolic state
KW - Nucleus
KW - Proliferation
KW - Transcription
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U2 - 10.1007/s00418-005-0085-x
DO - 10.1007/s00418-005-0085-x
M3 - Article
C2 - 16235044
AN - SCOPUS:30044435205
SN - 0948-6143
VL - 125
SP - 193
EP - 201
JO - Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie
JF - Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie
IS - 1-2
ER -