TY - JOUR
T1 - Cutting edge
T2 - IL-1β mediates the proangiogenic activity of osteopontin-activated human monocytes
AU - Naldini, Antonella
AU - Leali, Dana
AU - Pucci, Annalisa
AU - Morena, Emilia
AU - Carraro, Fabio
AU - Nico, Beatrice
AU - Ribatti, Domenico
AU - Presta, Marco
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-Iβ up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-α, IL-8, and IL-6, a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1 Ab, thus indicating that this cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1β mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1β.
AB - Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-Iβ up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-α, IL-8, and IL-6, a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1 Ab, thus indicating that this cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1β mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1β.
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M3 - Article
C2 - 16982859
AN - SCOPUS:33749151344
SN - 0022-1767
VL - 177
SP - 4267
EP - 4270
JO - Journal of Immunology
JF - Journal of Immunology
IS - 7
ER -