Cutting edge: IL-1β mediates the proangiogenic activity of osteopontin-activated human monocytes

Antonella Naldini, Dana Leali, Annalisa Pucci, Emilia Morena, Fabio Carraro, Beatrice Nico, Domenico Ribatti, Marco Presta

Research output: Contribution to journalArticlepeer-review


Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-Iβ up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-α, IL-8, and IL-6, a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1 Ab, thus indicating that this cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1β mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1β.

Original languageEnglish
Pages (from-to)4267-4270
Number of pages4
JournalJournal of Immunology
Issue number7
Publication statusPublished - Oct 1 2006

ASJC Scopus subject areas

  • Immunology


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