Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells

D. Ghigo; D. Geromin; C. Franchino; R. Todde; C. Priotto; C. Costamagna; M. Arese; G. Garbarino; G. P. Pescarmona; A. Bosia

doi:10.1007/BF00807330

Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells

D. Ghigo, D. Geromin, C. Franchino, R. Todde, C. Priotto, C. Costamagna, M. Arese, G. Garbarino, G. P. Pescarmona, A. Bosia

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycin-elicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.

Original language	English
Pages (from-to)	277-281
Number of pages	5
Journal	Amino Acids
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/BF00807330
Publication status	Published - 1996

Keywords

1-chloro-2,4-dinitrobenzene
Amino acids
Endothelial cells
Glutathione
N-(2-mercaptopropionyl)-glycine
Nitric oxide
Polyomavirus

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry
Endocrinology

Access to Document

10.1007/BF00807330

Cite this

@article{457f4c60dba8416d88ba3fabfb069a0b,

title = "Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells",

abstract = "The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycin-elicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.",

keywords = "1-chloro-2,4-dinitrobenzene, Amino acids, Endothelial cells, Glutathione, N-(2-mercaptopropionyl)-glycine, Nitric oxide, Polyomavirus",

author = "D. Ghigo and D. Geromin and C. Franchino and R. Todde and C. Priotto and C. Costamagna and M. Arese and G. Garbarino and Pescarmona, {G. P.} and A. Bosia",

year = "1996",

doi = "10.1007/BF00807330",

language = "English",

volume = "10",

pages = "277--281",

journal = "Amino Acids",

issn = "0939-4451",

publisher = "Springer Wien",

number = "3",

}

TY - JOUR

T1 - Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells

AU - Ghigo, D.

AU - Geromin, D.

AU - Franchino, C.

AU - Todde, R.

AU - Priotto, C.

AU - Costamagna, C.

AU - Arese, M.

AU - Garbarino, G.

AU - Pescarmona, G. P.

AU - Bosia, A.

PY - 1996

Y1 - 1996

N2 - The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycin-elicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.

AB - The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycin-elicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.

KW - 1-chloro-2,4-dinitrobenzene

KW - Amino acids

KW - Endothelial cells

KW - Glutathione

KW - N-(2-mercaptopropionyl)-glycine

KW - Nitric oxide

KW - Polyomavirus

UR - http://www.scopus.com/inward/record.url?scp=9344229755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9344229755&partnerID=8YFLogxK

U2 - 10.1007/BF00807330

DO - 10.1007/BF00807330

M3 - Article

C2 - 24178542

AN - SCOPUS:9344229755

SN - 0939-4451

VL - 10

SP - 277

EP - 281

JO - Amino Acids

JF - Amino Acids

IS - 3

ER -

Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this