Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection

Paola Mantegani; Federica Piana; Laura Galli; Paolo Scarpellini; Luigi Codecasa; Adriano Lazzarin; Daniela Chillo; Claudio Fortis

Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection

Paola Mantegani, Federica Piana, Laura Galli, Paolo Scarpellini, Luigi Codecasa, Adriano Lazzarin, Daniela Chillo, Claudio Fortis

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity, Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T cell-based IFN-γ release assays. The aim of this study was to compare a RD1-based in-house ELISPOT-IFN-γ assay with a commercial (T-SPOT.TB) assay for the diagnosis of tuberculosis infection. The results showed an almost complete concordance between the two assays, confirming that our restricted but highly selected pool of peptides is sufficient to detect tuberculosis infection.

Original language	English
Pages (from-to)	291-294
Number of pages	4
Journal	New Microbiologica
Volume	30
Issue number	3
Publication status	Published - Jul 2007

Keywords

Bacillus Calmette-Guérin
ELISPOT
IFN
Tuberculosis

ASJC Scopus subject areas

Microbiology (medical)
Microbiology

Cite this

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AU - Mantegani, Paola

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AU - Galli, Laura

AU - Scarpellini, Paolo

AU - Codecasa, Luigi

AU - Lazzarin, Adriano

AU - Chillo, Daniela

AU - Fortis, Claudio

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AB - Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity, Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T cell-based IFN-γ release assays. The aim of this study was to compare a RD1-based in-house ELISPOT-IFN-γ assay with a commercial (T-SPOT.TB) assay for the diagnosis of tuberculosis infection. The results showed an almost complete concordance between the two assays, confirming that our restricted but highly selected pool of peptides is sufficient to detect tuberculosis infection.

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Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection

Abstract

Keywords

ASJC Scopus subject areas

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