TY - GEN
T1 - Comparative Proteomics for the Evaluation of Protein Expression and Modifications in Neurodegenerative Diseases
AU - Conti, Antonio
AU - Alessio, Massimo
PY - 2015
Y1 - 2015
N2 - Together with hypothesis-driven approaches, high-throughput differential proteomic analysis performed primarily not only in human cerebrospinal fluid and serum but also on protein content of other tissues (blood cells, muscles, peripheral nerves, etc.) has been used in the last years to investigate neurodegenerative diseases. Even if the goal for these analyses was mainly the discovery of neurodegenerative disorders biomarkers, the characterization of specific posttranslational modifications (PTMs) and the differential protein expression resulted in being very informative to better define the pathological mechanisms. In this chapter are presented and discussed the positive aspects and challenges of the outcomes of some of our investigations on neurological and neurodegenerative disease, in order to highlight the important role of protein PTMs studies in proteomics-based approaches.
AB - Together with hypothesis-driven approaches, high-throughput differential proteomic analysis performed primarily not only in human cerebrospinal fluid and serum but also on protein content of other tissues (blood cells, muscles, peripheral nerves, etc.) has been used in the last years to investigate neurodegenerative diseases. Even if the goal for these analyses was mainly the discovery of neurodegenerative disorders biomarkers, the characterization of specific posttranslational modifications (PTMs) and the differential protein expression resulted in being very informative to better define the pathological mechanisms. In this chapter are presented and discussed the positive aspects and challenges of the outcomes of some of our investigations on neurological and neurodegenerative disease, in order to highlight the important role of protein PTMs studies in proteomics-based approaches.
KW - Amyotrophic lateral sclerosis
KW - Biomarker
KW - Cerebrospinal fluid
KW - Mass spectrometry
KW - Parkinson's disease
KW - Posttranslational modifications
KW - Two-dimensional electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=84973423130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973423130&partnerID=8YFLogxK
U2 - 10.1016/bs.irn.2015.05.004
DO - 10.1016/bs.irn.2015.05.004
M3 - Conference contribution
C2 - 26315764
AN - SCOPUS:84973423130
SN - 9780128014806
VL - 121
T3 - International Review of Neurobiology
SP - 117
EP - 152
BT - International Review of Neurobiology
PB - Academic Press Inc.
ER -