Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line

Flora Cimmino; Daniela Spano; Mario Capasso; Nicola Zambrano; Roberta Russo; Massimo Zollo; Achille Iolascon

doi:10.1021/pr060701g

Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line

Flora Cimmino, Daniela Spano, Mario Capasso, Nicola Zambrano, Roberta Russo, Massimo Zollo, Achille Iolascon

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Abstract

Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

Original language	English
Pages (from-to)	2550-2564
Number of pages	15
Journal	Journal of Proteome Research
Volume	6
Issue number	7
DOIs	https://doi.org/10.1021/pr060701g
Publication status	Published - Jul 2007

Keywords

2D-DIGE
All-trans-retinoic acid
Cell line
Human neuroblastoma
LAN-5
Neuroblastoma

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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10.1021/pr060701g

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abstract = "Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.",

keywords = "2D-DIGE, All-trans-retinoic acid, Cell line, Human neuroblastoma, LAN-5, Neuroblastoma",

author = "Flora Cimmino and Daniela Spano and Mario Capasso and Nicola Zambrano and Roberta Russo and Massimo Zollo and Achille Iolascon",

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AU - Cimmino, Flora

AU - Spano, Daniela

AU - Capasso, Mario

AU - Zambrano, Nicola

AU - Russo, Roberta

AU - Zollo, Massimo

AU - Iolascon, Achille

PY - 2007/7

Y1 - 2007/7

N2 - Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

AB - Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

KW - 2D-DIGE

KW - All-trans-retinoic acid

KW - Cell line

KW - Human neuroblastoma

KW - LAN-5

KW - Neuroblastoma

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Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line

Abstract

Keywords

ASJC Scopus subject areas

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