TY - JOUR
T1 - Comparative analysis of DNA repair in stem and nonstem glioma cell cultures
AU - Ropolo, Monica
AU - Daga, Antonio
AU - Griffero, Fabrizio
AU - Foresta, Mara
AU - Casartelli, Gianluigi
AU - Zunino, Annalisa
AU - Poggi, Alessandro
AU - Cappedi, Enrico
AU - Zona, Gianluigi
AU - Spaziante, Renato
AU - Corte, Giorgio
AU - Frosina, Guido
PY - 2009/3/1
Y1 - 2009/3/1
N2 - It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. We have examined DNA repair in five stem and nonstem glioma cell lines. The population doubling time was significantly increased in stem compared with nonstem cells, and enhanced activation of Chk1 and Chk2 kinases was observed in untreated CD133+ compared with CD133 cells. Neither DNA base excision or single-strand break repair nor resolution of pH2AX nuclear foci were increased in CD133+ compared with CD133- cells. We conclude that glioma stem cells display elongated cell cycle and enhanced basal activation of checkpoint proteins that might contribute to their radioresistance, whereas enhanced DNA repair is not a common feature of these cells.
AB - It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. We have examined DNA repair in five stem and nonstem glioma cell lines. The population doubling time was significantly increased in stem compared with nonstem cells, and enhanced activation of Chk1 and Chk2 kinases was observed in untreated CD133+ compared with CD133 cells. Neither DNA base excision or single-strand break repair nor resolution of pH2AX nuclear foci were increased in CD133+ compared with CD133- cells. We conclude that glioma stem cells display elongated cell cycle and enhanced basal activation of checkpoint proteins that might contribute to their radioresistance, whereas enhanced DNA repair is not a common feature of these cells.
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U2 - 10.1158/1541-7786.MCR-08-0409
DO - 10.1158/1541-7786.MCR-08-0409
M3 - Article
C2 - 19276180
AN - SCOPUS:62549142665
SN - 1541-7786
VL - 7
SP - 383
EP - 392
JO - Molecular Cancer Research
JF - Molecular Cancer Research
IS - 3
ER -