Comparative analysis of ANDE 6C rapid DNA analysis system and traditional methods

Michele Ragazzo, Stefano Melchiorri, Laura Manzo, Valeria Errichiello, Giulio Puleri, Fabio Nicastro, Emiliano Giardina

Research output: Contribution to journalArticlepeer-review


Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This “swab in— profile out” method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equipment (ANDE) 6C system as a typing method for reference samples according to the ISO/IEC 17025 standard. Here, we report the evaluation of the validity and reproducibility of results by the comparison of the genetic profiles generated by the ANDE 6C System with those generated by standard technologies. A quantity of 104 buccal swabs were analyzed both through the ANDE 6C technology and the traditional method (DNA extraction and quantification, amplification and separation by capillary electrophoresis). Positive typing was observed in 97% of cases for ANDE 6C technology with only three buccal swabs failing to reveal interpretable signals. Concordance was determined by comparing the allele calls generated by ANDE 6C and conventional technology. Comparison of 2800 genotypes revealed a concordance rate of 99.96%. These results met the ISO/IEC 17025 requirements, enabling us to receive the accreditation for this method. Finally, rapid technology has certainly reached a level of reliability which has made its use in laboratories of forensic genetics a reality.

Original languageEnglish
Article number582
Issue number5
Publication statusPublished - May 2020


  • ANDE chip
  • Electropherogram
  • Forensic DNA typing
  • Rapid DNA analysis
  • Short tandem repeats

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


Dive into the research topics of 'Comparative analysis of ANDE 6C rapid DNA analysis system and traditional methods'. Together they form a unique fingerprint.

Cite this