TY - JOUR
T1 - Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region
AU - Pergolizzi, Rossana
AU - Appierto, Valentina
AU - Bosetti, Alessandro
AU - Debellis, GianLuca
AU - Rovida, Ermanna
AU - Biunno, Ida
PY - 1996/2/12
Y1 - 1996/2/12
N2 - To contribute to the isolation of genes within the q24-qter region of the human X chromosome, we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.
AB - To contribute to the isolation of genes within the q24-qter region of the human X chromosome, we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.
KW - Automated DNA sequencing
KW - Homology search
KW - Human X chromosome
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U2 - 10.1016/0378-1119(95)00741-5
DO - 10.1016/0378-1119(95)00741-5
M3 - Article
C2 - 8654957
AN - SCOPUS:0030022213
SN - 0378-1119
VL - 168
SP - 267
EP - 270
JO - Gene
JF - Gene
IS - 2
ER -