Chlorpromazine inhibits nitric oxide-mediated increase in intracellular cGMP in a mouse teratocarcinoma cell line

M. Fratelli; R. Delgado; M. Zinetti; G. Galli; Y. Rolland; P. Ghezzi

doi:10.1007/BF02032570

Chlorpromazine inhibits nitric oxide-mediated increase in intracellular cGMP in a mouse teratocarcinoma cell line

M. Fratelli, R. Delgado, M. Zinetti, G. Galli, Y. Rolland, P. Ghezzi

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

Chlorpromazine is a phenothiazine with a structure similar to that of methylene blue. Since methylene blue is a well known inhibitor of nitric oxideinduced cyclic GMP accumulation, we investigated whether chlorpromazine had the same effect. Cyclic GMP accumulation, induced in a mouse teratocarcinoma cell line (P19) by sodium nitroprusside (a nitric oxide releasing agent), was inhibited by both methylene blue (IC₅₀ 0.34 μM) and chlorpromazine (IC₅₀ 35 μM). Chlorpromazine's action was probably directed specifically at soluble guanylate cyclase, since the drug had no effect on ADP-ribosylation in rat hippocampus, another nitric oxide-affected, but cGMP-independent event.

Original language	English
Pages (from-to)	287-290
Number of pages	4
Journal	Inflammation Research
Volume	44
Issue number	7
DOIs	https://doi.org/10.1007/BF02032570
Publication status	Published - Jul 1995

Keywords

ADP-Ribosylation
Methylene blue
P19 cells
Phenothiazines
Soluble guanylyl cyclase

ASJC Scopus subject areas

Toxicology
Pharmacology (medical)
Immunology
Cell Biology

Access to Document

10.1007/BF02032570

Cite this

@article{38a3ae9fc97b45ba8c287f0331131565,

title = "Chlorpromazine inhibits nitric oxide-mediated increase in intracellular cGMP in a mouse teratocarcinoma cell line",

abstract = "Chlorpromazine is a phenothiazine with a structure similar to that of methylene blue. Since methylene blue is a well known inhibitor of nitric oxideinduced cyclic GMP accumulation, we investigated whether chlorpromazine had the same effect. Cyclic GMP accumulation, induced in a mouse teratocarcinoma cell line (P19) by sodium nitroprusside (a nitric oxide releasing agent), was inhibited by both methylene blue (IC50 0.34 μM) and chlorpromazine (IC50 35 μM). Chlorpromazine's action was probably directed specifically at soluble guanylate cyclase, since the drug had no effect on ADP-ribosylation in rat hippocampus, another nitric oxide-affected, but cGMP-independent event.",

keywords = "ADP-Ribosylation, Methylene blue, P19 cells, Phenothiazines, Soluble guanylyl cyclase",

author = "M. Fratelli and R. Delgado and M. Zinetti and G. Galli and Y. Rolland and P. Ghezzi",

year = "1995",

month = jul,

doi = "10.1007/BF02032570",

language = "English",

volume = "44",

pages = "287--290",

journal = "Inflammation Research",

issn = "1023-3830",

publisher = "Birkhauser Verlag Basel",

number = "7",

}

TY - JOUR

T1 - Chlorpromazine inhibits nitric oxide-mediated increase in intracellular cGMP in a mouse teratocarcinoma cell line

AU - Fratelli, M.

AU - Delgado, R.

AU - Zinetti, M.

AU - Galli, G.

AU - Rolland, Y.

AU - Ghezzi, P.

PY - 1995/7

Y1 - 1995/7

N2 - Chlorpromazine is a phenothiazine with a structure similar to that of methylene blue. Since methylene blue is a well known inhibitor of nitric oxideinduced cyclic GMP accumulation, we investigated whether chlorpromazine had the same effect. Cyclic GMP accumulation, induced in a mouse teratocarcinoma cell line (P19) by sodium nitroprusside (a nitric oxide releasing agent), was inhibited by both methylene blue (IC50 0.34 μM) and chlorpromazine (IC50 35 μM). Chlorpromazine's action was probably directed specifically at soluble guanylate cyclase, since the drug had no effect on ADP-ribosylation in rat hippocampus, another nitric oxide-affected, but cGMP-independent event.

AB - Chlorpromazine is a phenothiazine with a structure similar to that of methylene blue. Since methylene blue is a well known inhibitor of nitric oxideinduced cyclic GMP accumulation, we investigated whether chlorpromazine had the same effect. Cyclic GMP accumulation, induced in a mouse teratocarcinoma cell line (P19) by sodium nitroprusside (a nitric oxide releasing agent), was inhibited by both methylene blue (IC50 0.34 μM) and chlorpromazine (IC50 35 μM). Chlorpromazine's action was probably directed specifically at soluble guanylate cyclase, since the drug had no effect on ADP-ribosylation in rat hippocampus, another nitric oxide-affected, but cGMP-independent event.

KW - ADP-Ribosylation

KW - Methylene blue

KW - P19 cells

KW - Phenothiazines

KW - Soluble guanylyl cyclase

UR - http://www.scopus.com/inward/record.url?scp=0029082450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029082450&partnerID=8YFLogxK

U2 - 10.1007/BF02032570

DO - 10.1007/BF02032570

M3 - Article

C2 - 8564525

AN - SCOPUS:0029082450

SN - 1023-3830

VL - 44

SP - 287

EP - 290

JO - Inflammation Research

JF - Inflammation Research

IS - 7

ER -

Chlorpromazine inhibits nitric oxide-mediated increase in intracellular cGMP in a mouse teratocarcinoma cell line

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this