Changes of mitochondria and relocation of the apoptosis-inducing factor during apoptosis

A. Ivana Scovassi, Cristiana Soldani, Paola Veneroni, Maria Grazia Bottone, Carlo Pellicciari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

During apoptosis, apoptosis-inducing factor (AIF) is released from the mitochondrial intermembrane space to the cytosol and to the nucleus. We analyzed AIF in HeLa cells driven to apoptosis by either etoposide or actinomycin D, and we observed changes in the structure and function of mitochondria as well as the translocation of cytochrome c and AIF from mitochondria to the nucleus in early apoptosis. In cells with fragmented chromatin (i.e., in late apoptosis), the immunolabeling for AIF appeared to be distinct from chromatin, being mainly confined to mitochondria.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages12-17
Number of pages6
Volume1171
DOIs
Publication statusPublished - Aug 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • AIF
  • Apoptosis
  • Cytochrome c
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Changes of mitochondria and relocation of the apoptosis-inducing factor during apoptosis'. Together they form a unique fingerprint.

Cite this