TY - JOUR
T1 - Caveolin-1 is essential for glimepiride-induced insulin secretion in the pancreatic βTC-6 cell line
AU - Puddu, A.
AU - Salani, B.
AU - Cordera, R.
AU - Viviani, G. L.
AU - Maggi, D.
PY - 2008/10/17
Y1 - 2008/10/17
N2 - The KATP channels play a pivotal role in the complex mechanism of insulin secretion. KATP channels represent the target of sulphonylureas, a class of drugs widely used in type 2 diabetes to stimulate insulin secretion. We previously showed that caveolin-1 depletion impairs action of the sulphonylurea glimepiride in human endothelial cells. The aim of this work was to investigate the possible role of caveolin-1 in glimepiride-induced insulin secretion. Caveolin-1 was depleted using siRNA method in the pancreatic βTC-6 cell line. Then stimulation of insulin secretion was performed with different secretagogues (glucose, KCl, and glimepiride). Here, we show that βTC-6 caveolin-1 depleted cells maintained high rate of insulin secretion after KCl, but not after glucose and glimepiride stimulation. Moreover, we find a direct interaction between caveolin-1 and Kir6.2, one of the KATP channel subunit. These results demonstrate that Cav-1 plays a critical role for glucose and sulfonylurea-stimulated insulin secretion.
AB - The KATP channels play a pivotal role in the complex mechanism of insulin secretion. KATP channels represent the target of sulphonylureas, a class of drugs widely used in type 2 diabetes to stimulate insulin secretion. We previously showed that caveolin-1 depletion impairs action of the sulphonylurea glimepiride in human endothelial cells. The aim of this work was to investigate the possible role of caveolin-1 in glimepiride-induced insulin secretion. Caveolin-1 was depleted using siRNA method in the pancreatic βTC-6 cell line. Then stimulation of insulin secretion was performed with different secretagogues (glucose, KCl, and glimepiride). Here, we show that βTC-6 caveolin-1 depleted cells maintained high rate of insulin secretion after KCl, but not after glucose and glimepiride stimulation. Moreover, we find a direct interaction between caveolin-1 and Kir6.2, one of the KATP channel subunit. These results demonstrate that Cav-1 plays a critical role for glucose and sulfonylurea-stimulated insulin secretion.
KW - Caveolin-1
KW - Insulin secretion
KW - K
KW - Sulphonylurea
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UR - http://www.scopus.com/inward/citedby.url?scp=50249120587&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.08.003
DO - 10.1016/j.bbrc.2008.08.003
M3 - Article
C2 - 18703018
AN - SCOPUS:50249120587
SN - 0006-291X
VL - 375
SP - 235
EP - 237
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -